Fabrication of Porous Extracellular Matrix Scaffolds from Human Adipose Tissue

Adipose tissue is found over the whole body and easily obtained in large quantities with minimal risk by a common surgical operation, liposuction. Although liposuction was originally intended for the removal of undesired adipose tissue, it may provide an ideal material for tissue engineering scaffol...

Full description

Saved in:
Bibliographic Details
Published in:Tissue engineering. Part C, Methods Methods, 2010-06, Vol.16 (3), p.387-396
Main Authors: Choi, Ji Suk, Yang, Hyun-Jin, Kim, Beob Soo, Kim, Jae Dong, Lee, Sang Hoon, Lee, Eun Kyu, Park, Kinam, Cho, Yong Woo, Lee, Hee Young
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adipose Tissue - metabolism
Adipose tissues
Adult
Base Sequence
Biocompatible Materials
Biomedical materials
Body fat
Cells, Cultured
Cellular biology
Collagen - metabolism
DNA Primers
Extracellular Matrix
Female
Humans
Microscopy, Electron, Scanning
Physiological aspects
Porosity
Properties
Regenerative medicine
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells - cytology
Stem Cells - metabolism
Tissue Engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adipose tissue is found over the whole body and easily obtained in large quantities with minimal risk by a common surgical operation, liposuction. Although liposuction was originally intended for the removal of undesired adipose tissue, it may provide an ideal material for tissue engineering scaffolds. Here we present novel, porous scaffolds prepared from human adipose tissues. The scaffolds were fabricated in a variety of macroscopic shapes such as round dishes, squares, hollow tubes, and beads. The microscopic inner porous structure was controlled by the freezing temperature, with a decrease in pore size as the freezing temperature decreased. The scaffold prepared from human adipose tissue contains extracellular matrix components including collagen. Preliminary in vitro studies showed that human adipose-derived stem cells attached to a human extracellular matrix scaffold and proliferated. This scaffold based on human adipose tissue holds great promise for many clinical applications in regenerative medicine, particularly in patients requiring soft-tissue regeneration.
ISSN:1937-3384
1937-3392
DOI:10.1089/ten.tec.2009.0276