HLA-DQA1, AB0, and AMEL genotyping of biological material with biochips

A method for genotyping of biological material for AB0, HLA-DQA1, and AMEL loci is described. The method utilizes allele-specific SNP typing with th e help of hydrogel biochip technology. Amplified fluorescentlylabeled fragments of genes were hybridized with SNP-specific DNA probes immobilized on a...

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Bibliographic Details
Published in:Molecular biology (New York) 2010-06, Vol.44 (3), p.401-406
Main Authors: Fesenko, D. O, Mityaeva, O. N, Nasedkina, T. V, Rubtsov, P. M, Lysov, Yu. P, Zasedatelev, A. S
Format: Article
Language:English
Subjects:
Biochemistry
Biology
Biomedical and Life Sciences
Gene loci
Genomics. Transcriptomics
Genotype & phenotype
Human Genetics
Integrated circuits
Life Sciences
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Summary:A method for genotyping of biological material for AB0, HLA-DQA1, and AMEL loci is described. The method utilizes allele-specific SNP typing with th e help of hydrogel biochip technology. Amplified fluorescentlylabeled fragments of genes were hybridized with SNP-specific DNA probes immobilized on a biochip. The alleles of the gene in a sample were determined according to the distribution of the fluorescent signal. The minimal amount of biological material required for the assay is 100 pg of DNA. The biochip assay was used to analyze 442 DNA specimens of the Eastern Slavic population of Russia and to determine the allelic frequencies of AB0 and HLA-DQA1 loci. The feasibility of genotyping the biological traces of investigative value, such as cigarette butts, sweat traces on paper, and swabs of the lip of the glass, was demonstrated. This assay is applicable in forensic studies. The significance of identification for the used loci is 99.6%.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893310030076