Genetic characterization of green bean (Phaseolus vulgaris) genotypes from eastern Turkey

Green bean genotypes collected from eastern Turkey were characterized using simple sequence repeat (SSR) markers and morphological traits. Among 12 SSR markers, 10 produced successful amplifications and revealed DNA polymorphisms that were subsequently used to assess genetic relatedness of the genot...

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Bibliographic Details
Published in:Genetics and molecular research 2009-01, Vol.8 (3), p.880-887
Main Authors: Sarıkamış, G, Yaşar, F, Bakır, M, Kazan, K, Ergül, A
Format: Article
Language:English
Subjects:
Alleles
Base Pairing - genetics
beans
genetic distance
genetic markers
genetic polymorphism
genetic variation
Genotype
Geography
heterozygosity
Heterozygote
microsatellite repeats
Phaseolus - genetics
Phaseolus vulgaris
Phylogeny
Quantitative Trait, Heritable
Turkey
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Summary:Green bean genotypes collected from eastern Turkey were characterized using simple sequence repeat (SSR) markers and morphological traits. Among 12 SSR markers, 10 produced successful amplifications and revealed DNA polymorphisms that were subsequently used to assess genetic relatedness of the genotypes. Based on the number of alleles generated and the probability of identity values, the most informative SSR loci were PVGLND5, PVMEIG, PV-ag001, and PV-ag004. Probably, due to the inbreeding nature of beans, the heterozygosity observed within genotypes was low at most of the SSR loci. The UPGMA dendrogram constructed based on the SSR data yielded two major clusters. The overall genetic distance was around 98%, among the genotypes. This information can be used to help select Turkish green bean lines.
ISSN:1676-5680
1676-5680
DOI:10.4238/vol8-3gmr605