Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of Saussurea medusa

Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75 +/- 0.41 and 6.4 +/- 0.31 mg l(-1). Production of jaceosidin and hi...

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Bibliographic Details
Published in:Biotechnology letters 2005, Vol.27 (2), p.91-95
Main Authors: Fu, C.X, Zhao, D.X, Huang, Y, Ma, F.S
Format: Article
Language:English
Subjects:
Aggregates
Biological and medical sciences
biological production
Biomass
biosynthesis
Biotechnology
Biotechnology - methods
Callus
Cell Culture Techniques
Cell Line
cell lines
Cell Proliferation
Cell Size
Cell Survival
cell suspension culture
Cellular
Correlation
Culture
flavones
Flavones - biosynthesis
Flavonoids
Flavonoids - biosynthesis
Fundamental and applied biological sciences. Psychology
medicinal plants
Oriental traditional medicine
Q1
Q2
Saussurea
Saussurea - cytology
Saussurea - metabolism
Saussurea medusa
Suspension culture
traditional Chinese medicine
Viability
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Summary:Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75 +/- 0.41 and 6.4 +/- 0.31 mg l(-1). Production of jaceosidin and hispidulin correlated with the sizes of compact callus aggregates (CCA) and cellular viability. In the red cell line, the sizes of CCA were predominantly of 2-4 mm diameter and accounted for 64% biomass. This line had a sustained cell viability over 10 successive sub-cultures.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-004-6934-1