Elicitor-induced nitric oxide burst is essential for triggering catharanthine synthesis in Catharanthus roseus suspension cells

Elicitor prepared from the cell walls of Penicillium citrinum induced multiple responses in Catharanthus roseus suspension cells, including rapid generation of nitric oxide (NO), sequentially followed by enhancement of catharanthine production by C. roseus cells. Elicitor-induced catharanthine biosy...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2005-04, Vol.67 (1), p.40-44
Main Authors: Xu, Maojun, Dong, Jufang
Format: Article
Language:English
Subjects:
Benzoates
Biological and medical sciences
Biosynthesis
Biotechnology
Catharanthus - drug effects
Catharanthus - metabolism
Catharanthus roseus
Cell walls
Enzyme Inhibitors - pharmacology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Imidazoles
Methods. Procedures. Technologies
Miscellaneous
Nitric oxide
Nitric Oxide - metabolism
Nitric Oxide Synthase - antagonists & inhibitors
Nitric-oxide synthase
Nitroprusside - metabolism
Penicillium - chemistry
Penicillium citrinum
Plant cells and fungal cells
Q1
Q2
Respiratory Burst
sodium nitroprusside
Suspension culture
Thiourea - analogs & derivatives
Thiourea - pharmacology
Vinca Alkaloids - biosynthesis
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Summary:Elicitor prepared from the cell walls of Penicillium citrinum induced multiple responses in Catharanthus roseus suspension cells, including rapid generation of nitric oxide (NO), sequentially followed by enhancement of catharanthine production by C. roseus cells. Elicitor-induced catharanthine biosynthesis was blocked by NO-specific scavenger 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and nitric oxide synthase (NOS) inhibitor S,S'-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea (PBITU). PBITU also strongly inhibited elicitor-induced NO generation by C. roseus suspension cells. The inhibiting effect of PBITU on elicitor-induced catharanthine production was reversed by external application of NO via the NO-donor sodium nitroprusside. The results strongly suggested that NO, generated by NOS or NOS-like enzymes in C. roseus suspension cells when treated with the fungal elicitor, was essential for triggering catharanthine synthesis.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-004-1737-9