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Antimicrobial susceptibility testing of rapidly growing mycobacteria by microdilution - Experience of a tertiary care centre
Purpose: The objective of the study was to perform antimicrobial susceptibility testing of rapidly growing mycobacteria (RGM) isolated from various clinically suspected cases of extrapulmonary tuberculosis, from January 2007 to April 2008, at a tertiary care centre in Mumbai. Materials and Methods:...
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Published in: | Indian journal of medical microbiology 2010-01, Vol.28 (1), p.48-50 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Purpose: The objective of the study was to perform antimicrobial
susceptibility testing of rapidly growing mycobacteria (RGM) isolated
from various clinically suspected cases of extrapulmonary tuberculosis,
from January 2007 to April 2008, at a tertiary care centre in Mumbai.
Materials and Methods: The specimens were processed for microscopy and
culture using the standard procedures. Minimum inhibitory
concentrations (MIC) were determined by broth microdilution, using
Sensititre CA MHBT. Susceptibility testing was also carried out on
Mueller Hinton agar by the Kirby Bauer disc diffusion method. Results:
Of the 1062 specimens received for mycobacterial cultures, 104 (9.79%)
grew mycobacteria. Of the mycobacterial isolates, six (5.76%) were
rapid growers. M. abscessus and M. chelonae appeared to be resistant
organisms, with M. chelonae showing intermediate resistance to amikacin
and minocycline. However, all the six isolates showed sensitivity to
vancomycin and gentamicin by the disc diffusion test. Also all three
isolates of M. abscessus were sensitive to piperacillin and
erythromycin. Further studies are required to test their sensitivity to
these four antimicrobials by using the microbroth dilution test, before
they can be prescribed to patients. Conclusions: We wish to emphasize
that reporting of rapidly growing mycobacteria from clinical settings,
along with their sensitivity patterns, is an absolute need of the hour. |
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ISSN: | 0255-0857 1998-3646 |
DOI: | 10.4103/0255-0857.58729 |