Genome-wide survey of protein kinases required for cell cycle progression

Cycles of protein phosphorylation are fundamental in regulating the progression of the eukaryotic cell through its division cycle. Here we test the complement of Drosophila protein kinases (kinome) for cell cycle functions after gene silencing by RNAmediated interference. We observed cell cycle dysf...

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Bibliographic Details
Published in:Nature 2004-12, Vol.432 (7020), p.980-987
Main Authors: Bettencourt-Dias, M, Giet, R, Sinka, R, Mazumdar, A, Lock, W.G, Balloux, F, Zafiropoulos, P.J, Yamaguchi, S, Winter, S, Carthew, R.W
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell cycle
Cell Cycle - genetics
Cell Cycle - physiology
Cell cycle, cell proliferation
Cell physiology
Cell Proliferation
Cytokinesis
Drosophila
Drosophila melanogaster
Drosophila melanogaster - cytology
Drosophila melanogaster - enzymology
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Enzymes
Eukaryotes
Fundamental and applied biological sciences. Psychology
G2 Phase
gene silencing
Genome
Genomics
Humanities and Social Sciences
microtubules
mitosis
Mitosis - physiology
Molecular and cellular biology
multidisciplinary
Mutation - genetics
Nutritional Status
protein kinases
Protein Kinases - genetics
Protein Kinases - metabolism
protein phosphorylation
Proteins
RNA Interference
S Phase
Science
Science (multidisciplinary)
Signal Transduction
Spindle Apparatus - physiology
Stress, Physiological - genetics
Stress, Physiological - physiopathology
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Summary:Cycles of protein phosphorylation are fundamental in regulating the progression of the eukaryotic cell through its division cycle. Here we test the complement of Drosophila protein kinases (kinome) for cell cycle functions after gene silencing by RNAmediated interference. We observed cell cycle dysfunction upon downregulation of 80 out of 228 protein kinases, including most kinases that are known to regulate the division cycle. We find new enzymes with cell cycle functions; some of these have family members already known to phosphorylate microtubules, actin or their associated proteins. Additionally, depletion of several signalling kinases leads to specific mitotic aberrations, suggesting novel roles for familiar enzymes. The survey reveals the interdigitation of systems that monitor cellular physiology, cell size, cellular stress and signalling processes with the basic cell cycle regulatory machinery.
ISSN:0028-0836
1476-4687
DOI:10.1038/nature03160