Approach to hydrophilic interaction chromatography column selection: Application to neurotransmitters analysis

This paper presents the comparison between 12 hydrophilic interaction liquid chromatography columns that are commercially available. The main factors influencing the retention and selectivity toward 12 neurotransmitters for these different HILIC systems have been studied. For additional information,...

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Bibliographic Details
Published in:Journal of Chromatography A 2010-04, Vol.1217 (18), p.3091-3104
Main Authors: Chirita, Raluca-Ioana, West, Caroline, Finaru, Adriana-Luminita, Elfakir, Claire
Format: Article
Language:English
Subjects:
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Catecholamines
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Fused core silica
HILIC
Neurotransmitters
Non peptidic neurotransmitters, polyamines
Other biological molecules
Other chromatographic methods
Retention behaviour
Stationary phases classification
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Summary:This paper presents the comparison between 12 hydrophilic interaction liquid chromatography columns that are commercially available. The main factors influencing the retention and selectivity toward 12 neurotransmitters for these different HILIC systems have been studied. For additional information, the retention data have been analyzed statistically by factor analysis. Principal component analyses (PCA) were calculated to evidence different separation behaviour between the stationary phases, based on the retention data measured for three compound classes: anionic acidic compounds, cationic basic compounds and zwitterionic amino acids. Finally, a generic procedure is suggested for optimization of HILIC analyses, depending on the ionization state of the analytes.
ISSN:0021-9673
DOI:10.1016/j.chroma.2010.03.001