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Approach to hydrophilic interaction chromatography column selection: Application to neurotransmitters analysis
This paper presents the comparison between 12 hydrophilic interaction liquid chromatography columns that are commercially available. The main factors influencing the retention and selectivity toward 12 neurotransmitters for these different HILIC systems have been studied. For additional information,...
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Published in: | Journal of Chromatography A 2010-04, Vol.1217 (18), p.3091-3104 |
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container_end_page | 3104 |
container_issue | 18 |
container_start_page | 3091 |
container_title | Journal of Chromatography A |
container_volume | 1217 |
creator | Chirita, Raluca-Ioana West, Caroline Finaru, Adriana-Luminita Elfakir, Claire |
description | This paper presents the comparison between 12 hydrophilic interaction liquid chromatography columns that are commercially available. The main factors influencing the retention and selectivity toward 12 neurotransmitters for these different HILIC systems have been studied. For additional information, the retention data have been analyzed statistically by factor analysis. Principal component analyses (PCA) were calculated to evidence different separation behaviour between the stationary phases, based on the retention data measured for three compound classes: anionic acidic compounds, cationic basic compounds and zwitterionic amino acids. Finally, a generic procedure is suggested for optimization of HILIC analyses, depending on the ionization state of the analytes. |
doi_str_mv | 10.1016/j.chroma.2010.03.001 |
format | article |
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The main factors influencing the retention and selectivity toward 12 neurotransmitters for these different HILIC systems have been studied. For additional information, the retention data have been analyzed statistically by factor analysis. Principal component analyses (PCA) were calculated to evidence different separation behaviour between the stationary phases, based on the retention data measured for three compound classes: anionic acidic compounds, cationic basic compounds and zwitterionic amino acids. 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Psychology</subject><subject>Fused core silica</subject><subject>HILIC</subject><subject>Neurotransmitters</subject><subject>Non peptidic neurotransmitters, polyamines</subject><subject>Other biological molecules</subject><subject>Other chromatographic methods</subject><subject>Retention behaviour</subject><subject>Stationary phases classification</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kE1r3DAQhn1IoWmafxCILyGn3Y4kr2X1UAghH4VAD23OYnY8jrXYkit5C_vvo41Djj0JRs880vsWxYWAtQBRf9utqY9hxLWEPAK1BhAnxSmAFCtTa_W5-JLSLg81aHla-JtpigGpL-dQ9oc2hql3g6PS-Zkj0uyCLxfjHF4iTv2hpDDsR18mHvjt_nuZJXkH3-Ds8byPYY7o0-jmrEklehwOyaWvxacOh8Tn7-dZ8Xx_9-f2cfX06-Hn7c3TijZCzataajbdtlEKQElDbWNyuC0q7Nig6jrQBipJGlrebGrTbklBxVRXwkiSpM6K68Wbw_3dc5rt6BLxMKDnsE9WV1VttBBNJquFpBhSitzZKboR48EKsMdG7c4u-e2xUQvK5vLy2tX7A5gIhy6HJZc-dqWs1aYxR_3lwnUYLL7EzDz_ziIFopG6kTITPxaCcx__HEebyLEnbl3M_do2uP9_5RVFMZw_</recordid><startdate>20100430</startdate><enddate>20100430</enddate><creator>Chirita, Raluca-Ioana</creator><creator>West, Caroline</creator><creator>Finaru, Adriana-Luminita</creator><creator>Elfakir, Claire</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20100430</creationdate><title>Approach to hydrophilic interaction chromatography column selection: Application to neurotransmitters analysis</title><author>Chirita, Raluca-Ioana ; West, Caroline ; Finaru, Adriana-Luminita ; Elfakir, Claire</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-627e9fb83300329cd89101ba3afe9a3ff079042c70de5569dbc304ec64192c2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytical chemistry</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Catecholamines</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fused core silica</topic><topic>HILIC</topic><topic>Neurotransmitters</topic><topic>Non peptidic neurotransmitters, polyamines</topic><topic>Other biological molecules</topic><topic>Other chromatographic methods</topic><topic>Retention behaviour</topic><topic>Stationary phases classification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chirita, Raluca-Ioana</creatorcontrib><creatorcontrib>West, Caroline</creatorcontrib><creatorcontrib>Finaru, Adriana-Luminita</creatorcontrib><creatorcontrib>Elfakir, Claire</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chirita, Raluca-Ioana</au><au>West, Caroline</au><au>Finaru, Adriana-Luminita</au><au>Elfakir, Claire</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Approach to hydrophilic interaction chromatography column selection: Application to neurotransmitters analysis</atitle><jtitle>Journal of Chromatography A</jtitle><date>2010-04-30</date><risdate>2010</risdate><volume>1217</volume><issue>18</issue><spage>3091</spage><epage>3104</epage><pages>3091-3104</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>This paper presents the comparison between 12 hydrophilic interaction liquid chromatography columns that are commercially available. 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subjects | Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Catecholamines Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Fundamental and applied biological sciences. Psychology Fused core silica HILIC Neurotransmitters Non peptidic neurotransmitters, polyamines Other biological molecules Other chromatographic methods Retention behaviour Stationary phases classification |
title | Approach to hydrophilic interaction chromatography column selection: Application to neurotransmitters analysis |
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