RGMb controls aggregation and migration of Neogenin-positive cells in vitro and in vivo

The proliferation, migration and differentiation of dentate gyrus stem and precursor cells have aroused keen interest. Neogenin and RGMb are expressed in non-overlapping compartments of the developing dentate gyrus. While Neogenin is expressed in migrating and proliferating dentate precursors, RGMb...

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Bibliographic Details
Published in:Molecular and cellular neuroscience 2010-02, Vol.43 (2), p.222-231
Main Authors: Conrad, Sabine, Stimpfle, Fabian, Montazeri, Sonia, Oldekamp, Judit, Seid, Karin, Alvarez-Bolado, Gonzalo, Skutella, Thomas
Format: Article
Language:English
Subjects:
Animals
Cell Line, Transformed
Cell Movement - physiology
Dentate gyrus development
Electroporation - methods
Embryo, Mammalian
Fluoresceins
Gene Expression Regulation, Developmental - physiology
Green Fluorescent Proteins - genetics
Hippocampus - cytology
Homeodomain Proteins - metabolism
Humans
Immunoprecipitation
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mice
Mice, Inbred C57BL
Migration
Neogenin
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neurons - physiology
Organ Culture Techniques
RGMb
Stem Cells - physiology
Transfection - methods
Tumor Suppressor Proteins - metabolism
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Summary:The proliferation, migration and differentiation of dentate gyrus stem and precursor cells have aroused keen interest. Neogenin and RGMb are expressed in non-overlapping compartments of the developing dentate gyrus. While Neogenin is expressed in migrating and proliferating dentate precursors, RGMb is localized in structures bordering the developing dentate, such as cornus ammonis cells and Cajal–Retzius cells in the marginal zone including the hippocampal fissure. Co-immunoprecipitation and binding assays indicate a strong physical interaction. In vitro and in vivo migration of dentate neuroepithelial cells is abolished by RGMb, and cell adhesion is reduced when cells expressing Neogenin comes into contact with cells expressing RGMb. Ectopic expression of RGMb in organotypic slice cultures and after in utero electroporation in the hippocampus modifies precursor cell migration. Our results imply that Neogenin-RGMb interaction might be involved in neuronal migration in the dentate gyrus.
ISSN:1044-7431
1095-9327
DOI:10.1016/j.mcn.2009.11.003