Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals

The number of biotechnology-based pharmaceuticals in the late-stage pipeline has been increasing more than ever. As a result, there is an enhanced demand for more efficient and cost-effective processes. During the last years, the upstream technology for the production of biopharmaceuticals has been...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 2010-04, Vol.1217 (16), p.2296-2305
Main Authors: Rosa, P.A.J., Ferreira, I.F., Azevedo, A.M., Aires-Barros, M.R.
Format: Article
Language:English
Subjects:
Aqueous two-phase systems (ATPSs)
Biopharmaceuticals
Downstream processing
Manufacturing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The number of biotechnology-based pharmaceuticals in the late-stage pipeline has been increasing more than ever. As a result, there is an enhanced demand for more efficient and cost-effective processes. During the last years, the upstream technology for the production of biopharmaceuticals has been considerably improved. Continuous discoveries in molecular biology and genetics, combined with new advances in media and feed development, have significantly increased the production titres. In order to keep up this gain, it is now essential to design new, as well as to improve the existing downstream processes that remain an unresolved bottleneck. This review evaluates several alternatives to the currently established platforms for the downstream processing biopharmaceuticals, with main focus on aqueous two-phase extraction.
ISSN:0021-9673
DOI:10.1016/j.chroma.2009.11.034