PCR multiplex for detection of Salmonella Enteritidis, Typhi and Typhimurium and occurrence in poultry meat

• Published in: International journal of food microbiology 2010-04, Vol.139 (1), p.15-22
• Main Authors: de Freitas, Camila Guimarães, Santana, Ângela Patrícia, da Silva, Patrícia Helena Caldeira, Gonçalves, Vítor Salvador Picão, Barros, Márcia de Aguiar Ferreira, Torres, Fernando Araripe Gonçalves, Murata, Luci Sayori, Perecmanis, Simone
• Format: Article
• Language: English
• Subjects: Animals; bacterial contamination; Biological and medical sciences; Carcasses; DNA, Bacterial - analysis; Enteritidis; food analysis; food contamination; Food industries; Food Microbiology; food pathogens; food processing; food processing quality; Fundamental and applied biological sciences. Psychology; General aspects; Meat - microbiology; meat composition; meat quality; Methods of analysis, processing and quality control, regulation, standards; microbial detection; microbiological quality; Multiplex PCR; polymerase chain reaction; Polymerase Chain Reaction - methods; Poultry; Poultry - microbiology; poultry meat; rapid methods; Salmonella; Salmonella - genetics; Salmonella - isolation & purification; Salmonella enteritidis; Salmonella enteritidis - genetics; Salmonella enteritidis - isolation & purification; Salmonella Food Poisoning - prevention & control; Salmonella typhi; Salmonella typhi - genetics; Salmonella typhi - isolation & purification; Salmonella typhimurium; Salmonella typhimurium - genetics; Salmonella typhimurium - isolation & purification; serotypes; Serotyping - methods; Typhi; Typhimurium
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2010.02.007

The occurrence of foodborne diseases is increasing throughout the world. Bacteria of the genus Salmonella are responsible for food poisoning and, in some cases, may be fatal. The aim of this study was to adapt the multiplex PCR technique (mPCR) on the rapid and direct identification of the presence of Salmonella sp. as well as serotypes Enteritidis, Typhi and Typhimurium in poultry carcasses ( n = 127) and viscera ( n = 73). The implementation of the standard technique using positive controls was successfully adapted. The results of Salmonella sp. detection in refrigerated viscera showed that the mPCR was able to detect Salmonella genus in 2.74% of these samples. Traditional microbiological analysis also identified the same positive samples for Salmonella sp. but was not able to differentiate the serotype. The serotype Enteritidis was detected by mPCR in 1.37% of the samples. Our conclusion was that the mPCR was able to detect the presence of these bacteria in a short period of time and enabled the identification of serotype Enteritidis in one of the samples found positive for Salmonella sp.