Structural elucidation of the repeat unit in highly branched acidic exopolysaccharides produced by nitrogen fixing Burkholderia

Burkholderia kururiensis, strain M130, an endophytic diazotrophic bacterium isolated from rice roots, produces acetylated acidic exopolysaccharides which can be separated by anion exchange chromatography. These were characterized by nuclear magnetic resonance spectroscopy, methylation analysis and S...

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Bibliographic Details
Published in:Glycobiology (Oxford) 2010-03, Vol.20 (3), p.338-347
Main Authors: Hallack, Leticia F, Passos, Daniel S, Mattos, Katherine A, Agrellos, Orlando A, Jones, Christopher, Mendonça-Previato, Lucia, Previato, Jose O, Todeschini, Adriane R
Format: Article
Language:English
Subjects:
Burkholderia
Burkholderia - metabolism
Carbohydrate Sequence
endophyte
exopolysaccharide
Molecular Sequence Data
Nitrogen - metabolism
Nitrogen Fixation
nitrogen fixing
NMR spectroscopy
Oryza sativa
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - isolation & purification
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Summary:Burkholderia kururiensis, strain M130, an endophytic diazotrophic bacterium isolated from rice roots, produces acetylated acidic exopolysaccharides which can be separated by anion exchange chromatography. These were characterized by nuclear magnetic resonance spectroscopy, methylation analysis and Smith degradation. The exopolysaccharides eluted with 0.5 M NaCl were produced when the bacterium was grown in a medium containing mannitol as the sole carbon source, and showed to be a mixture of two different polymers, composed of hepta or octasaccharide repeat units, consistent with following structure: Formula The ability of diazotrophic Burkholderia to produce two exopolysaccharides that differ by the presence of a terminal glucosyl residue provides insight into polysaccharide function with potentially significant biological consequences in the endophytic-host plant interaction.
ISSN:0959-6658
1460-2423
DOI:10.1093/glycob/cwp181