chick ovomucoid gene contains at least six intervening sequences

A 15-kilobase pair Eco RI chick DNA fragment, containing both the termination codon UGA and the 5′-portion of the structural ovomucoid gene, has been cloned in λ phage Charon 4 A by in vitro packaging. Restriction mapping and electron microscopic analyses of this cloned DNA have revealed that the st...

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Bibliographic Details
Published in:Nature (London) 1979-03, Vol.278 (5702), p.323-327
Main Authors: Catterall, J.F, Stein, J.P, Lai, E.C, Woo, S.L.C, Dugaiczyk, A, Mace, M.L, Means, A.R, O'Malley, B.W
Format: Article
Language:English
Subjects:
animal breeding
animal science
Animals
Base Sequence
Chickens
DNA Restriction Enzymes - metabolism
DNA, Recombinant
Egg Proteins - genetics
Genes
Humanities and Social Sciences
livestock
Molecular Weight
multidisciplinary
Nucleic Acid Hybridization
Ovomucin - genetics
Plasmids
Science
Science (multidisciplinary)
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Summary:A 15-kilobase pair Eco RI chick DNA fragment, containing both the termination codon UGA and the 5′-portion of the structural ovomucoid gene, has been cloned in λ phage Charon 4 A by in vitro packaging. Restriction mapping and electron microscopic analyses of this cloned DNA have revealed that the structural ovomucoid gene sequences are separated by at least six intervening sequences.
ISSN:0028-0836
1476-4687
DOI:10.1038/278323a0