Thermodynamics of glucagon aggregation

Heats of dilution of concentrated glucagon solutions have been measured calorimetrically at 10 and 25 degrees C in 0.2 M potassium phosphate buffer of pH 10.6. Analysis of the data in terms of a monomer-trimer equilibrium gives the following thermodynamic parameters for the association reaction at 2...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Easton) 1979-04, Vol.18 (7), p.1176-1179
Main Authors: Johnson, Robert E, Hruby, Victor J, Rupley, John A
Format: Article
Language:English
Subjects:
Calorimetry
Glucagon
Macromolecular Substances
Thermodynamics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Heats of dilution of concentrated glucagon solutions have been measured calorimetrically at 10 and 25 degrees C in 0.2 M potassium phosphate buffer of pH 10.6. Analysis of the data in terms of a monomer-trimer equilibrium gives the following thermodynamic parameters for the association reaction at 25 degrees C: delta G degrees = 7.34 kcal/mol of trimer, delta H degrees = -31.2 kcal/mol, deltaS degrees = -80 cal/(K mol), deltaCp = 430 cal/(K mol). The sensitivity of heat of dilution data to the association constant and stoichiometry of the reaction is discussed.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00574a009