Thermodynamics of glucagon aggregation

Heats of dilution of concentrated glucagon solutions have been measured calorimetrically at 10 and 25 degrees C in 0.2 M potassium phosphate buffer of pH 10.6. Analysis of the data in terms of a monomer-trimer equilibrium gives the following thermodynamic parameters for the association reaction at 2...

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Published in:Biochemistry (Easton) 1979-04, Vol.18 (7), p.1176-1179
Main Authors: Johnson, Robert E, Hruby, Victor J, Rupley, John A
Format: Article
Language:English
Subjects:
Calorimetry
Glucagon
Macromolecular Substances
Thermodynamics
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Hruby, Victor J
Rupley, John A
description Heats of dilution of concentrated glucagon solutions have been measured calorimetrically at 10 and 25 degrees C in 0.2 M potassium phosphate buffer of pH 10.6. Analysis of the data in terms of a monomer-trimer equilibrium gives the following thermodynamic parameters for the association reaction at 25 degrees C: delta G degrees = 7.34 kcal/mol of trimer, delta H degrees = -31.2 kcal/mol, deltaS degrees = -80 cal/(K mol), deltaCp = 430 cal/(K mol). The sensitivity of heat of dilution data to the association constant and stoichiometry of the reaction is discussed.
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source ACS CRKN Legacy Archives
subjects Calorimetry
Glucagon
Macromolecular Substances
Thermodynamics
title Thermodynamics of glucagon aggregation
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