Amino acid sequences of the alpha and beta chains of adult hemoglobin of the brown lemur, Lemur fulvus fulvus

Globin prepared from hemoglobin of the brown lemur (Lemur fulvus fulvus) was separated into a and chains by chromatography on a CM 52 column. The S-aminoethylated α and β chains were each digested with trypsin and resulting peptides were isolated. The amino acid sequences of the tryptic peptides wer...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1979-03, Vol.85 (3), p.755-764
Main Authors: MAITA, Tetsuo, SETOGUCHI, Masayuki, MATSUDA, Genji, GOODMAN, Morris
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - analysis
Animals
Globins - analysis
Hemoglobins - analysis
Humans
Lemur - blood
Methods
Peptide Fragments - analysis
Strepsirhini - blood
Trypsin
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Summary:Globin prepared from hemoglobin of the brown lemur (Lemur fulvus fulvus) was separated into a and chains by chromatography on a CM 52 column. The S-aminoethylated α and β chains were each digested with trypsin and resulting peptides were isolated. The amino acid sequences of the tryptic peptides were established. The ordering of these peptides in the α and β chains was deduced from the homology of their amino acid sequences with that of human adult hemoglobin. The primary structure of brown lemur hemoglobin thus obtained differs from that of human hemoglobin in 15 amino acids in the α chain and 26 in the β chain.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a132389