Piezoelectric crystal immunosensors based on immobilized fullerene C60-antibodies

Fullerene C60-antibodies, e.g., C60-anti-human IgG and C60-anti-hemoglobin (Hb), coated piezoelectric crystals were prepared and applied in piezoelectric quartz crystal immunosensors for human IgG and hemoglobin, respectively. The immobilization of anti-IgG and anti-Hb onto fullerene C60 were studie...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2004-03, Vol.98 (2), p.180-187
Main Authors: Pan, Nai-Yu, Shih, Jeng-Shong
Format: Article
Language:English
Subjects:
Anti-hemoglobin
Anti-IgG
Antibodies
Biosensors
C60-anti-hemoglobin
C60-anti-human IgG
Crystals
Fullerene
Hemoglobin
Immobilization
Immobilized antibody
Immunoassays
Immunoglobulin G
Piezoelectric immunosensor
Piezoelectricity
Q1
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Summary:Fullerene C60-antibodies, e.g., C60-anti-human IgG and C60-anti-hemoglobin (Hb), coated piezoelectric crystals were prepared and applied in piezoelectric quartz crystal immunosensors for human IgG and hemoglobin, respectively. The immobilization of anti-IgG and anti-Hb onto fullerene C60 were studied with a C60-coated piezoelectric crystal detection system. The partially irreversible responses for these antibodies were observed by the desorption study, which implied that C60 could chemically react with these bio-species. Thus, immobilized C60-anti-IgG and C60-anti-Hb coating materials were successfully prepared and identified with FTIR spectrometry. The C60-anti-human IgG and C60-anti-Hb coated piezoelectric (PZ) quartz crystal immunosensors with homemade computer interfaces for signal acquisition and data processing were developed and applied for detection of human IgG and hemoglobin, respectively. The C60-antibody coated PZ Bio-Sensors for IgG and hemoglobin exhibited linear frequency responses to the concentrations of human IgG and hemoglobin with sensitivities of 1.25×10 2 Hz per decade (ΔHz/Δlog (mg/ml)) and 1.56×10 4 Hz/(mg/ml), respectively. Both C60-antibody coated PZ immunosensors showed detection limit of
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2003.09.034