Surface-Enhanced Raman Scattering Substrate Based on a Self-Assembled Monolayer for Use in Gene Diagnostics

The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-lab...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2003-11, Vol.75 (22), p.6196-6201
Main Authors: Culha, Mustafa, Stokes, David, Allain, Leonardo R, Vo-Dinh, Tuan
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
BRCA1 protein
BRCA1 Protein - genetics
Breast cancer
Chemistry
DNA probes
DNA Probes - analysis
DNA Probes - chemistry
DNA Probes - genetics
DNA, Single-Stranded - genetics
Exact sciences and technology
Genetic Testing - methods
Gynecology. Andrology. Obstetrics
Hexanols
Humans
Mammary gland diseases
Medical sciences
Molecular Structure
Q1
Rhodamines
Sensitivity and Specificity
Silver
Spectrometric and optical methods
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
Tumors
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Summary:The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH−(CH2)6-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microcopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0346003