Molecular assessment of genetic diversity in mung bean germplasm

RAPD profiles were used to identify the extent of diversity among 54 accessions of mung bean that included both improved and local land races. Out of the 40 primers screened, seven primers generated 174 amplification products with an average of 24.85 bands per primer. The RAPD profiles were analysed...

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Bibliographic Details
Published in:Journal of genetics 2008-04, Vol.87 (1), p.65-74
Main Authors: Lavanya, G. Roopa, Srivastava, Jyoti, Ranade, Shirish A.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Base Sequence
Biomedical and Life Sciences
DNA Primers - genetics
DNA, Plant - genetics
Evolutionary Biology
Fabaceae - genetics
Genetic Variation
Life Sciences
Microbial Genetics and Genomics
Plant Genetics and Genomics
Random Amplified Polymorphic DNA Technique
Research Article
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Summary:RAPD profiles were used to identify the extent of diversity among 54 accessions of mung bean that included both improved and local land races. Out of the 40 primers screened, seven primers generated 174 amplification products with an average of 24.85 bands per primer. The RAPD profiles were analysed for Jaccard’s similarity coefficients that was found to be in the range from 0 to 0.48, indicating the presence of wide range of genetic diversity at molecular level. Cluster analysis was carried out based on distances (1-similarity coefficient) using neighbour-joining method in Free Tree package. The dendrogram resolved all the accessions into two major clusters, I (with 11 accessions) and II (with 43 accessions). However, the cluster was further divided into four subclusters (II A with six, II B with nine, II C with 15 and II D with 13 accessions). The distribution of the accessions in different clusters and subclusters appears to be related to their performance in field conditions for 10 morphological traits that were scored. This study indicated that the RAPD profiles provide an easy and simple technique for preliminary genetic diversity assessment of mung bean accessions that may reflect morphological trait differences among them.
ISSN:0022-1333
0973-7731
DOI:10.1007/s12041-008-0009-3