Ultrasonic treatment for improved solution properties of a high-molecular weight exopolysaccharide produced by a medicinal fungus

High-power ultrasound (20 kHz) was applied to modify the physicochemical properties of a high-molecular weight (MW) exopolysaccharide (EPS) from mycelial culture of a medicinal fungus. At 35 W/cm 2 or higher ultrasound power, the apparent and intrinsic viscosities of EPS solution dropped by nearly 8...

Full description

Saved in:
Bibliographic Details
Published in:Bioresource technology 2010-07, Vol.101 (14), p.5517-5522
Main Authors: Wang, Zhao-Mei, Cheung, Yi-Ching, Leung, Po-Hong, Wu, Jian-Yong
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Line
Congo Red - pharmacology
Cordyceps sinensis
Culture
EPS
Exopolysaccharide
Fundamental and applied biological sciences. Psychology
Fungi
Fungi - metabolism
Humans
Hydrolysis
Microscopy, Atomic Force - methods
Molecular Weight
Polysaccharides
Polysaccharides - chemistry
Solubility
Sonication
Spectrophotometry, Infrared - methods
Temperature
Time Factors
Ultrasonic degradation
Ultrasonic treatment
Ultrasonics
Ultrasound
Viscosity
Viscosity and solubility
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:High-power ultrasound (20 kHz) was applied to modify the physicochemical properties of a high-molecular weight (MW) exopolysaccharide (EPS) from mycelial culture of a medicinal fungus. At 35 W/cm 2 or higher ultrasound power, the apparent and intrinsic viscosities of EPS solution dropped by nearly 85% within 10 min, and the water solubility was increased by more than fourfold. The ultrasonic treatment led to a notable reduction of the maximum MW and a more uniform MW distribution, but no significant change in the primary structure of the EPS molecules. In contrast, the intrinsic viscosity of EPS was reduced by only 20% in 1.0 M sulfuric acid at 50 °C for 9 h. Ultrasound was proven an effective and favorable means for improving the solution properties of high-MW bioactive polysaccharides in mild conditions.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2010.01.134