Turbidity and Color Correction in the Microtox Bioassay

We propose a new method of correcting for the effects of color and turbidity on bacterial light output measurements in the Microtox bioassay. Using our method, toxicity assessment involves clearly defined calculation factors, based on light loss measurements using a modified double-cuvette. An exist...

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Bibliographic Details
Published in:The Open environmental pollution & toxicology journal 2010-01, Vol.2, p.1-7
Main Authors: Ashworth, John, Nijenhuis, Erik, Glowacka, Bozena, Tran, Lang, Schenk-Watt, Lisa
Format: Article
Language:English
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Summary:We propose a new method of correcting for the effects of color and turbidity on bacterial light output measurements in the Microtox bioassay. Using our method, toxicity assessment involves clearly defined calculation factors, based on light loss measurements using a modified double-cuvette. An existing color-correction method requires additional spectrometer measurements and over-corrects for turbidity. For a range of clear, colored fluids our new method and the existing method gave similar, corrected EC50 values. Our new method needs no separate equipment and is likely to provide valid toxicity assessments of whole fluids, without any need for sample clarification. It accounted for > 90 % of the variance in loss of measured bacterial light caused by color and turbidity of a range of test fluids.
ISSN:1876-3979
DOI:10.2174/1876397901002010001