Promoter-Selective Properties of the TBP-Related Factor TRF1

The TATA-binding protein (TBP)-related factor 1 (TRF1) is expressed in a tissue-restricted fashion during Drosophila embryogenesis and may serve as a promoter-specific recognition factor that can replace TBP in regulating transcription. However, bona fide target promoters that would preferentially r...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2000-05, Vol.288 (5467), p.867-870
Main Authors: Holmes, Michael C., Tjian, Robert
Format: Article
Language:English
Subjects:
Animals
Apoptosis
B lymphocytes
Biological and medical sciences
Cell Line
Cell lines
Cells
DNA
DNA - metabolism
DNA Footprinting
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Drosophila
Drosophila - genetics
Drosophila Proteins
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes
Genes, Insect
Genes, Reporter
Genetic regulation
Genetic transcription
Individualized Instruction
Insect Proteins - genetics
Insects
Logical Thinking
Membrane Transport Proteins
Messenger RNA
Molecular and cellular biology
Molecular genetics
Promoter Regions, Genetic
Proteins
Recombinant Proteins - metabolism
Regulation
Ribonucleic acid
RNA
TATA Box Binding Protein-Like Proteins
TATA-Box Binding Protein
Transcription (Genetics)
Transcription Factor TFIIA
Transcription Factor TFIIB
Transcription Factor TFIID
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription Factors, TFII - metabolism
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Transfection
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Description
Summary:The TATA-binding protein (TBP)-related factor 1 (TRF1) is expressed in a tissue-restricted fashion during Drosophila embryogenesis and may serve as a promoter-specific recognition factor that can replace TBP in regulating transcription. However, bona fide target promoters that would preferentially respond to TRF1 have remained elusive. Polytene chromosome staining, chromatin immunoprecipitation, direct messenger RNA analysis, and transient co-transfection assays identified the Drosophila gene tudor as containing a TRF1-responsive promoter. Reconstituted in vitro transcription reactions and deoxyribonuclease I footprinting assays confirmed the ability of TRF1 to bind preferentially and direct transcription of the tudor gene from an alternate promoter. Thus, metazoans appear to have evolved gene-selective and tissue-specific components of the core transcription machinery to regulate gene expression.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.288.5467.867