Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia‐associated immunophenotypes

Background: The knowledge of normal marrow is mandatory to assess the malignant counterpart of normal cells and define leukemia‐associated immunophenotypes (LAIPs). In this study, the expression of a variety of antigens expressed in normal and postchemotherapy bone marrow (BM) was analyzed to provid...

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Published in:Cytometry. Part B, Clinical cytometry Clinical cytometry, 2008-01, Vol.74B (1), p.17-24
Main Authors: Olaru, D., Campos, L., Flandrin, P., Nadal, N., Duval, A., Chautard, S., Guyotat, D.
Format: Article
Language:English
Subjects:
Antineoplastic Agents - therapeutic use
Bone Marrow - drug effects
Bone Marrow - immunology
Bone Marrow - pathology
Child
Flow Cytometry - methods
Humans
Immunophenotyping
Leukemia, Myeloid, Acute - drug therapy
Leukemia, Myeloid, Acute - immunology
leukemia‐associated immunophenotype
Middle Aged
multiparameter flow cytometry
Neoplasm, Residual
normal bone marrow
Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy
Precursor Cell Lymphoblastic Leukemia-Lymphoma - immunology
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Summary:Background: The knowledge of normal marrow is mandatory to assess the malignant counterpart of normal cells and define leukemia‐associated immunophenotypes (LAIPs). In this study, the expression of a variety of antigens expressed in normal and postchemotherapy bone marrow (BM) was analyzed to provide a frame of reference for the identification of myeloid LAIPs. Methods: Multiparameter four‐ and six‐color flow cytometry was used to define antigen combinations totally absent or present at very minimal levels in marrow cells of normal individuals (n = 20) and patients receiving chemotherapy for acute lymphoblastic leukemia (n = 20). Immature (blast) cells were gated according to CD45/SSC properties. Fifty‐three acute myeloid leukemia (AML) samples were studied in six‐color combinations. Results: In six‐color flow cytometry, 47 phenotypes were totally absent from blast gate in all normal samples. Forty‐one other phenotypes were identified in less than 0.05% of blast cells. There was no difference between normal and postchemotherapy BMs. The four‐color panel allowed to identify only 30 phenotypes present at a frequency
ISSN:1552-4949
1552-4957
DOI:10.1002/cyto.b.20371