Loading…

Production of recombinant proteins in high-density insect cell cultures

The effect of the growth phase of Spodoptera frugiperda (Sf9) cells on the production of recombinant proteins (beta-galactosidase and glucocerebrosidase) was investigated. Cells infected with the recombinant Autographa californica nuclear polyhedrosis virus at the late exponential and stationary pha...

Full description

Saved in:

Bibliographic Details
Published in:	Biotechnology and bioengineering 1993-06, Vol.42 (2), p.235-239
Main Authors:	Reuveny, S, Kim, Y.J, Kemp, C.W, Shiloach, J
Format:	Article
Language:	English
Subjects:	ADN RECOMBINADO ADN RECOMBINE Animal cells BACULOVIRIDAE baculovirus BETA GALACTOSIDASA BETA GALACTOSIDASE Biological and medical sciences BIOREACTEUR BIORREACTORES Biotechnology CULTIVO DE CELULAS CULTURE DE CELLULE Eukaryotic cell cultures EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GLICOSIDASAS GLYCOSIDASE high density culture insect cells Lepidoptera MEDIO DE CULTIVO Methods. Procedures. Technologies MILIEU DE CULTURE Miscellaneous Noctuidae PATHOGENESE PATOGENESIS recombinant protein production SINTESIS DE PROTEINAS SPODOPTERA FRUGIPERDA SYNTHESE PROTEIQUE
Citations:	Items that this one cites Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c6281-169228d21fd39f41206729681bde89c02d86cf3e5b1816bf03b0672a407a757f3
cites	cdi_FETCH-LOGICAL-c6281-169228d21fd39f41206729681bde89c02d86cf3e5b1816bf03b0672a407a757f3
container_end_page	239
container_issue	2
container_start_page	235
container_title	Biotechnology and bioengineering
container_volume	42
creator	Reuveny, S Kim, Y.J Kemp, C.W Shiloach, J
description	The effect of the growth phase of Spodoptera frugiperda (Sf9) cells on the production of recombinant proteins (beta-galactosidase and glucocerebrosidase) was investigated. Cells infected with the recombinant Autographa californica nuclear polyhedrosis virus at the late exponential and stationary phases yielded low quantities of expressed protein. Highest enzyme yields were obtained using Sf9 cells from the early exponential phase (0.9 mg beta-galactosidase/10(6) cells and 1.7 micrograms glucocerebrosidase/10(6) cells). Infection of resuspension of cells collected from various phases of growth in fresh medium resulted in 75% restoration of maximal expression levels. This finding suggested either nutrient limitation or waste product accumulation as the cause of the decrease in productivity at the latter phases of growth. Further experiments revealed that the highest productivity levels could be obtained with cultures of Sf9 cells grown in a fermentor to a cell concentration of 4 X 10(6) mL-1. The medium needed to be replaced prior to infection with the recombinant virus and supplemented with a mixture of glucose, L-glutamine, and yeastolate ultrafiltrate
doi_str_mv	10.1002/bit.260420211
format	article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_745652099</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733452968</sourcerecordid><originalsourceid>FETCH-LOGICAL-c6281-169228d21fd39f41206729681bde89c02d86cf3e5b1816bf03b0672a407a757f3</originalsourceid><addsrcrecordid>eNqF0U1v1DAQBmALgehSOHLhgHJAcErx2I4_jrDAtlIFBVpxtBzHbg3ZpNiOYP89jjZaOJWTNdLjmVczCD0FfAIYk9dtyCeEY0YwAbiHVoCVqDFR-D5aYYx5TRtFjtCjlL6XUkjOH6IjkByIkmyFNhdx7CabwzhUo6-is-O2DYMZcnUbx-zCkKowVDfh-qbu3JBC3pU6OZsr6_q-slOfp-jSY_TAmz65J8t7jK4-vL9cn9bnnzZn6zfnteVEQg1cESI7Ar6jyjMgmAuiuIS2c1JZTDrJraeuaUECbz2m7SwMw8KIRnh6jF7t-5Z0PyeXst6GNCcxgxunpAVreEOwUv-XlLJmnl3kyzslcK4EaaDAeg9tHFOKzuvbGLYm7jRgPV9Dl2vowzWKf740ntqt6_7qZf0FvFiASdb0PprBhnRwTEKZOvcRe_Yr9G5391D99uzy3wRL4pCy-334aeIPzQUVjf72caPXX9jpO3bxWc-reLb33ozaXMcS5uqrYlQoougfir-3mw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16697251</pqid></control><display><type>article</type><title>Production of recombinant proteins in high-density insect cell cultures</title><source>Wiley-Blackwell Journals (Backfile Content)</source><creator>Reuveny, S ; Kim, Y.J ; Kemp, C.W ; Shiloach, J</creator><creatorcontrib>Reuveny, S ; Kim, Y.J ; Kemp, C.W ; Shiloach, J</creatorcontrib><description>The effect of the growth phase of Spodoptera frugiperda (Sf9) cells on the production of recombinant proteins (beta-galactosidase and glucocerebrosidase) was investigated. Cells infected with the recombinant Autographa californica nuclear polyhedrosis virus at the late exponential and stationary phases yielded low quantities of expressed protein. Highest enzyme yields were obtained using Sf9 cells from the early exponential phase (0.9 mg beta-galactosidase/10(6) cells and 1.7 micrograms glucocerebrosidase/10(6) cells). Infection of resuspension of cells collected from various phases of growth in fresh medium resulted in 75% restoration of maximal expression levels. This finding suggested either nutrient limitation or waste product accumulation as the cause of the decrease in productivity at the latter phases of growth. Further experiments revealed that the highest productivity levels could be obtained with cultures of Sf9 cells grown in a fermentor to a cell concentration of 4 X 10(6) mL-1. The medium needed to be replaced prior to infection with the recombinant virus and supplemented with a mixture of glucose, L-glutamine, and yeastolate ultrafiltrate</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260420211</identifier><identifier>PMID: 18612984</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>ADN RECOMBINADO ; ADN RECOMBINE ; Animal cells ; BACULOVIRIDAE ; baculovirus ; BETA GALACTOSIDASA ; BETA GALACTOSIDASE ; Biological and medical sciences ; BIOREACTEUR ; BIORREACTORES ; Biotechnology ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; Eukaryotic cell cultures ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GLICOSIDASAS ; GLYCOSIDASE ; high density culture ; insect cells ; Lepidoptera ; MEDIO DE CULTIVO ; Methods. Procedures. Technologies ; MILIEU DE CULTURE ; Miscellaneous ; Noctuidae ; PATHOGENESE ; PATOGENESIS ; recombinant protein production ; SINTESIS DE PROTEINAS ; SPODOPTERA FRUGIPERDA ; SYNTHESE PROTEIQUE</subject><ispartof>Biotechnology and bioengineering, 1993-06, Vol.42 (2), p.235-239</ispartof><rights>Copyright © 1993 John Wiley & Sons, Inc.</rights><rights>1993 INIST-CNRS</rights><rights>(c) 1993 John Wiley & Sons, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6281-169228d21fd39f41206729681bde89c02d86cf3e5b1816bf03b0672a407a757f3</citedby><cites>FETCH-LOGICAL-c6281-169228d21fd39f41206729681bde89c02d86cf3e5b1816bf03b0672a407a757f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.260420211$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.260420211$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1415,27923,27924,46048,46472</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4815131$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18612984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reuveny, S</creatorcontrib><creatorcontrib>Kim, Y.J</creatorcontrib><creatorcontrib>Kemp, C.W</creatorcontrib><creatorcontrib>Shiloach, J</creatorcontrib><title>Production of recombinant proteins in high-density insect cell cultures</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>The effect of the growth phase of Spodoptera frugiperda (Sf9) cells on the production of recombinant proteins (beta-galactosidase and glucocerebrosidase) was investigated. Cells infected with the recombinant Autographa californica nuclear polyhedrosis virus at the late exponential and stationary phases yielded low quantities of expressed protein. Highest enzyme yields were obtained using Sf9 cells from the early exponential phase (0.9 mg beta-galactosidase/10(6) cells and 1.7 micrograms glucocerebrosidase/10(6) cells). Infection of resuspension of cells collected from various phases of growth in fresh medium resulted in 75% restoration of maximal expression levels. This finding suggested either nutrient limitation or waste product accumulation as the cause of the decrease in productivity at the latter phases of growth. Further experiments revealed that the highest productivity levels could be obtained with cultures of Sf9 cells grown in a fermentor to a cell concentration of 4 X 10(6) mL-1. The medium needed to be replaced prior to infection with the recombinant virus and supplemented with a mixture of glucose, L-glutamine, and yeastolate ultrafiltrate</description><subject>ADN RECOMBINADO</subject><subject>ADN RECOMBINE</subject><subject>Animal cells</subject><subject>BACULOVIRIDAE</subject><subject>baculovirus</subject><subject>BETA GALACTOSIDASA</subject><subject>BETA GALACTOSIDASE</subject><subject>Biological and medical sciences</subject><subject>BIOREACTEUR</subject><subject>BIORREACTORES</subject><subject>Biotechnology</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>Eukaryotic cell cultures</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLICOSIDASAS</subject><subject>GLYCOSIDASE</subject><subject>high density culture</subject><subject>insect cells</subject><subject>Lepidoptera</subject><subject>MEDIO DE CULTIVO</subject><subject>Methods. Procedures. Technologies</subject><subject>MILIEU DE CULTURE</subject><subject>Miscellaneous</subject><subject>Noctuidae</subject><subject>PATHOGENESE</subject><subject>PATOGENESIS</subject><subject>recombinant protein production</subject><subject>SINTESIS DE PROTEINAS</subject><subject>SPODOPTERA FRUGIPERDA</subject><subject>SYNTHESE PROTEIQUE</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNqF0U1v1DAQBmALgehSOHLhgHJAcErx2I4_jrDAtlIFBVpxtBzHbg3ZpNiOYP89jjZaOJWTNdLjmVczCD0FfAIYk9dtyCeEY0YwAbiHVoCVqDFR-D5aYYx5TRtFjtCjlL6XUkjOH6IjkByIkmyFNhdx7CabwzhUo6-is-O2DYMZcnUbx-zCkKowVDfh-qbu3JBC3pU6OZsr6_q-slOfp-jSY_TAmz65J8t7jK4-vL9cn9bnnzZn6zfnteVEQg1cESI7Ar6jyjMgmAuiuIS2c1JZTDrJraeuaUECbz2m7SwMw8KIRnh6jF7t-5Z0PyeXst6GNCcxgxunpAVreEOwUv-XlLJmnl3kyzslcK4EaaDAeg9tHFOKzuvbGLYm7jRgPV9Dl2vowzWKf740ntqt6_7qZf0FvFiASdb0PprBhnRwTEKZOvcRe_Yr9G5391D99uzy3wRL4pCy-334aeIPzQUVjf72caPXX9jpO3bxWc-reLb33ozaXMcS5uqrYlQoougfir-3mw</recordid><startdate>19930620</startdate><enddate>19930620</enddate><creator>Reuveny, S</creator><creator>Kim, Y.J</creator><creator>Kemp, C.W</creator><creator>Shiloach, J</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19930620</creationdate><title>Production of recombinant proteins in high-density insect cell cultures</title><author>Reuveny, S ; Kim, Y.J ; Kemp, C.W ; Shiloach, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6281-169228d21fd39f41206729681bde89c02d86cf3e5b1816bf03b0672a407a757f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ADN RECOMBINADO</topic><topic>ADN RECOMBINE</topic><topic>Animal cells</topic><topic>BACULOVIRIDAE</topic><topic>baculovirus</topic><topic>BETA GALACTOSIDASA</topic><topic>BETA GALACTOSIDASE</topic><topic>Biological and medical sciences</topic><topic>BIOREACTEUR</topic><topic>BIORREACTORES</topic><topic>Biotechnology</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>Eukaryotic cell cultures</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLICOSIDASAS</topic><topic>GLYCOSIDASE</topic><topic>high density culture</topic><topic>insect cells</topic><topic>Lepidoptera</topic><topic>MEDIO DE CULTIVO</topic><topic>Methods. Procedures. Technologies</topic><topic>MILIEU DE CULTURE</topic><topic>Miscellaneous</topic><topic>Noctuidae</topic><topic>PATHOGENESE</topic><topic>PATOGENESIS</topic><topic>recombinant protein production</topic><topic>SINTESIS DE PROTEINAS</topic><topic>SPODOPTERA FRUGIPERDA</topic><topic>SYNTHESE PROTEIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reuveny, S</creatorcontrib><creatorcontrib>Kim, Y.J</creatorcontrib><creatorcontrib>Kemp, C.W</creatorcontrib><creatorcontrib>Shiloach, J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reuveny, S</au><au>Kim, Y.J</au><au>Kemp, C.W</au><au>Shiloach, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of recombinant proteins in high-density insect cell cultures</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>1993-06-20</date><risdate>1993</risdate><volume>42</volume><issue>2</issue><spage>235</spage><epage>239</epage><pages>235-239</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>The effect of the growth phase of Spodoptera frugiperda (Sf9) cells on the production of recombinant proteins (beta-galactosidase and glucocerebrosidase) was investigated. Cells infected with the recombinant Autographa californica nuclear polyhedrosis virus at the late exponential and stationary phases yielded low quantities of expressed protein. Highest enzyme yields were obtained using Sf9 cells from the early exponential phase (0.9 mg beta-galactosidase/10(6) cells and 1.7 micrograms glucocerebrosidase/10(6) cells). Infection of resuspension of cells collected from various phases of growth in fresh medium resulted in 75% restoration of maximal expression levels. This finding suggested either nutrient limitation or waste product accumulation as the cause of the decrease in productivity at the latter phases of growth. Further experiments revealed that the highest productivity levels could be obtained with cultures of Sf9 cells grown in a fermentor to a cell concentration of 4 X 10(6) mL-1. The medium needed to be replaced prior to infection with the recombinant virus and supplemented with a mixture of glucose, L-glutamine, and yeastolate ultrafiltrate</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18612984</pmid><doi>10.1002/bit.260420211</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext	fulltext
identifier	ISSN: 0006-3592
ispartof	Biotechnology and bioengineering, 1993-06, Vol.42 (2), p.235-239
issn	0006-3592 1097-0290
language	eng
recordid	cdi_proquest_miscellaneous_745652099
source	Wiley-Blackwell Journals (Backfile Content)
subjects	ADN RECOMBINADO ADN RECOMBINE Animal cells BACULOVIRIDAE baculovirus BETA GALACTOSIDASA BETA GALACTOSIDASE Biological and medical sciences BIOREACTEUR BIORREACTORES Biotechnology CULTIVO DE CELULAS CULTURE DE CELLULE Eukaryotic cell cultures EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GLICOSIDASAS GLYCOSIDASE high density culture insect cells Lepidoptera MEDIO DE CULTIVO Methods. Procedures. Technologies MILIEU DE CULTURE Miscellaneous Noctuidae PATHOGENESE PATOGENESIS recombinant protein production SINTESIS DE PROTEINAS SPODOPTERA FRUGIPERDA SYNTHESE PROTEIQUE
title	Production of recombinant proteins in high-density insect cell cultures
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T23%3A50%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Production%20of%20recombinant%20proteins%20in%20high-density%20insect%20cell%20cultures&rft.jtitle=Biotechnology%20and%20bioengineering&rft.au=Reuveny,%20S&rft.date=1993-06-20&rft.volume=42&rft.issue=2&rft.spage=235&rft.epage=239&rft.pages=235-239&rft.issn=0006-3592&rft.eissn=1097-0290&rft.coden=BIBIAU&rft_id=info:doi/10.1002/bit.260420211&rft_dat=%3Cproquest_cross%3E733452968%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c6281-169228d21fd39f41206729681bde89c02d86cf3e5b1816bf03b0672a407a757f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=16697251&rft_id=info:pmid/18612984&rfr_iscdi=true