Activated platelets increase proliferation and protein synthesis of human dental pulp-derived cells

Agis H, Stampfl B, Watzek G, Gruber R. Activated platelets increase proliferation and protein synthesis of human dental pulp–derived cells. International Endodontic Journal, 43, 115–124, 2010. Aim  To evaluate the impact of activated platelets on the mitogenic expansion of human dental pulp–derived...

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Published in:International endodontic journal 2010-02, Vol.43 (2), p.115-124
Main Authors: Agis, H., Stampfl, B., Watzek, G., Gruber, R.
Format: Article
Language:English
Subjects:
AKT/MAPK pathways
Biological Factors
Blood Platelets - metabolism
Calcium Hydroxide - pharmacology
cell proliferation
Cell Proliferation - drug effects
Cells, Cultured
Culture Media, Conditioned - pharmacology
Cytokines - physiology
Dental Pulp - cytology
Dental Pulp - drug effects
Dental Pulp - enzymology
Dentistry
Escherichia coli
human dental pulp-derived cells
Humans
inflammatory cytokines
Lipopolysaccharides - pharmacology
LPS
Membrane Proteins - pharmacology
Mitogen-Activated Protein Kinases - metabolism
Phosphatidylinositol 3-Kinases - metabolism
Platelet Activation
protein synthesis
Proto-Oncogene Proteins c-akt - drug effects
Proto-Oncogene Proteins c-akt - metabolism
Root Canal Filling Materials - pharmacology
Second Messenger Systems - drug effects
Second Messenger Systems - physiology
Signal Transduction - drug effects
Signal Transduction - physiology
Statistics, Nonparametric
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Summary:Agis H, Stampfl B, Watzek G, Gruber R. Activated platelets increase proliferation and protein synthesis of human dental pulp–derived cells. International Endodontic Journal, 43, 115–124, 2010. Aim  To evaluate the impact of activated platelets on the mitogenic expansion of human dental pulp–derived cells (DPC) in vitro. Methodology  The effect of supernatants released from activated platelets (PRS) and the corresponding platelet membranes (MEM) on proliferation and protein synthesis of DPC was evaluated. The contributions of the phosphoinositide 3‐kinase (PI3K)/AKT and mitogen‐activated protein kinase (MAPK) pathways to the response of DPC were assessed using kinase inhibitors. Also examined was whether the presence of calcium hydroxide or the inflammatory mediators tumour necrosis factor alpha, interleukin‐1, interleukin‐6 and lipopolysaccharide of Escherichia coli modulated the expansion of DPC. Results  Physiologic concentrations of PRS and MEM stimulated proliferation and protein synthesis by 18.4‐fold (P 
ISSN:0143-2885
1365-2591
DOI:10.1111/j.1365-2591.2009.01650.x