Loading…
Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodology
Theaflavins were synthesized from tea polyphenols extracted from green tea using an immobilized polyphenol oxidase system. To optimize the production of theaflavins, response surface methodology was applied to determine the effects of five critical variables and their mutual interactions on theaflav...
Saved in:
| Published in: | Biotechnology letters 2005-02, Vol.27 (4), p.269-274 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c491t-7135f652c2d78c35fcf463c06c89a9d39260e339fd690aa22f429c962a16aaff3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c491t-7135f652c2d78c35fcf463c06c89a9d39260e339fd690aa22f429c962a16aaff3 |
| container_end_page | 274 |
| container_issue | 4 |
| container_start_page | 269 |
| container_title | Biotechnology letters |
| container_volume | 27 |
| creator | TU, You-Ying XU, Xin-Qing XIA, Hui-Long WATANABE, Naoharu |
| description | Theaflavins were synthesized from tea polyphenols extracted from green tea using an immobilized polyphenol oxidase system. To optimize the production of theaflavins, response surface methodology was applied to determine the effects of five critical variables and their mutual interactions on theaflavin biosynthesis at five levels. A total of 52 individual experiments were performed and a statistical model predicted that the highest theaflavin concentration was 0.766 mg ml(-1) at optimized conditions. Using these optimal parameters under experimental conditions in three independent replicates, the average value of the biosynthesized theaflavin concentration reached 0.75 +/- 0.017 mg ml(-1) and matched the value predicted by the model. |
| doi_str_mv | 10.1007/s10529-004-8292-4 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_745693719</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>6429864</sourcerecordid><originalsourceid>FETCH-LOGICAL-c491t-7135f652c2d78c35fcf463c06c89a9d39260e339fd690aa22f429c962a16aaff3</originalsourceid><addsrcrecordid>eNp90U2LFDEQBuAgijuu_gAvEgT11JqvTjrHZfELFvai55BJJztZOkmb6hZ6Tv50s8zAggdPVVSeKggvQq8p-UgJUZ-Akp7pjhDRDUyzTjxBO9or3kml5FO0I1TQrheaXaAXAPeEEK2Ieo4uGhKMCr1Df27nJaZ4tEssGZeAl4O3YbK_Y8b7WGDLbQARcKgl4cVbPJdpmw8-lwnwCjHfYZtxTKns4xSPfsQ-H7fkMWyw-NQeR1w9zCVDm601WOdx8suhjGUqd9tL9CzYCfyrc71EP798_nH9rbu5_fr9-uqmc0LTpVOU90H2zLFRDa71LgjJHZFu0FaPXDNJPOc6jFITaxkLgmmnJbNUWhsCv0QfTnfnWn6tHhaTIjg_TTb7soJRopeaK6qbfP9fKZUYKOdDg2__gfdlrbn9wiguBil43zdET8jVAlB9MHONydbNUGIeUjSnFE1L0TykaETbeXM-vO6THx83zrE18O4MLDg7hWqzi_DopGRq6An_C1hGp2k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734864355</pqid></control><display><type>article</type><title>Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodology</title><source>Springer Nature</source><creator>TU, You-Ying ; XU, Xin-Qing ; XIA, Hui-Long ; WATANABE, Naoharu</creator><creatorcontrib>TU, You-Ying ; XU, Xin-Qing ; XIA, Hui-Long ; WATANABE, Naoharu</creatorcontrib><description>Theaflavins were synthesized from tea polyphenols extracted from green tea using an immobilized polyphenol oxidase system. To optimize the production of theaflavins, response surface methodology was applied to determine the effects of five critical variables and their mutual interactions on theaflavin biosynthesis at five levels. A total of 52 individual experiments were performed and a statistical model predicted that the highest theaflavin concentration was 0.766 mg ml(-1) at optimized conditions. Using these optimal parameters under experimental conditions in three independent replicates, the average value of the biosynthesized theaflavin concentration reached 0.75 +/- 0.017 mg ml(-1) and matched the value predicted by the model.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-004-8292-4</identifier><identifier>PMID: 15742149</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Biflavonoids - chemical synthesis ; Biological and medical sciences ; Biosynthesis ; Biotechnology ; Catechin - chemical synthesis ; Catechol Oxidase - chemistry ; Combinatorial Chemistry Techniques - methods ; Computer Simulation ; Enzymes ; Enzymes, Immobilized - chemistry ; Flavonoids - chemistry ; Fundamental and applied biological sciences. Psychology ; Green tea ; Immobilized enzymes ; Mathematical models ; Models, Chemical ; Optimization ; Phenols - chemistry ; Plant Extracts - chemistry ; Polyphenols ; Q1 ; Statistical analysis ; Statistical models ; Tea ; Tea - chemistry ; theaflavins</subject><ispartof>Biotechnology letters, 2005-02, Vol.27 (4), p.269-274</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-7135f652c2d78c35fcf463c06c89a9d39260e339fd690aa22f429c962a16aaff3</citedby><cites>FETCH-LOGICAL-c491t-7135f652c2d78c35fcf463c06c89a9d39260e339fd690aa22f429c962a16aaff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16627850$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15742149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TU, You-Ying</creatorcontrib><creatorcontrib>XU, Xin-Qing</creatorcontrib><creatorcontrib>XIA, Hui-Long</creatorcontrib><creatorcontrib>WATANABE, Naoharu</creatorcontrib><title>Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodology</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>Theaflavins were synthesized from tea polyphenols extracted from green tea using an immobilized polyphenol oxidase system. To optimize the production of theaflavins, response surface methodology was applied to determine the effects of five critical variables and their mutual interactions on theaflavin biosynthesis at five levels. A total of 52 individual experiments were performed and a statistical model predicted that the highest theaflavin concentration was 0.766 mg ml(-1) at optimized conditions. Using these optimal parameters under experimental conditions in three independent replicates, the average value of the biosynthesized theaflavin concentration reached 0.75 +/- 0.017 mg ml(-1) and matched the value predicted by the model.</description><subject>Biflavonoids - chemical synthesis</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Catechin - chemical synthesis</subject><subject>Catechol Oxidase - chemistry</subject><subject>Combinatorial Chemistry Techniques - methods</subject><subject>Computer Simulation</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Flavonoids - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Green tea</subject><subject>Immobilized enzymes</subject><subject>Mathematical models</subject><subject>Models, Chemical</subject><subject>Optimization</subject><subject>Phenols - chemistry</subject><subject>Plant Extracts - chemistry</subject><subject>Polyphenols</subject><subject>Q1</subject><subject>Statistical analysis</subject><subject>Statistical models</subject><subject>Tea</subject><subject>Tea - chemistry</subject><subject>theaflavins</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp90U2LFDEQBuAgijuu_gAvEgT11JqvTjrHZfELFvai55BJJztZOkmb6hZ6Tv50s8zAggdPVVSeKggvQq8p-UgJUZ-Akp7pjhDRDUyzTjxBO9or3kml5FO0I1TQrheaXaAXAPeEEK2Ieo4uGhKMCr1Df27nJaZ4tEssGZeAl4O3YbK_Y8b7WGDLbQARcKgl4cVbPJdpmw8-lwnwCjHfYZtxTKns4xSPfsQ-H7fkMWyw-NQeR1w9zCVDm601WOdx8suhjGUqd9tL9CzYCfyrc71EP798_nH9rbu5_fr9-uqmc0LTpVOU90H2zLFRDa71LgjJHZFu0FaPXDNJPOc6jFITaxkLgmmnJbNUWhsCv0QfTnfnWn6tHhaTIjg_TTb7soJRopeaK6qbfP9fKZUYKOdDg2__gfdlrbn9wiguBil43zdET8jVAlB9MHONydbNUGIeUjSnFE1L0TykaETbeXM-vO6THx83zrE18O4MLDg7hWqzi_DopGRq6An_C1hGp2k</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>TU, You-Ying</creator><creator>XU, Xin-Qing</creator><creator>XIA, Hui-Long</creator><creator>WATANABE, Naoharu</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20050201</creationdate><title>Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodology</title><author>TU, You-Ying ; XU, Xin-Qing ; XIA, Hui-Long ; WATANABE, Naoharu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-7135f652c2d78c35fcf463c06c89a9d39260e339fd690aa22f429c962a16aaff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biflavonoids - chemical synthesis</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Catechin - chemical synthesis</topic><topic>Catechol Oxidase - chemistry</topic><topic>Combinatorial Chemistry Techniques - methods</topic><topic>Computer Simulation</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Flavonoids - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Green tea</topic><topic>Immobilized enzymes</topic><topic>Mathematical models</topic><topic>Models, Chemical</topic><topic>Optimization</topic><topic>Phenols - chemistry</topic><topic>Plant Extracts - chemistry</topic><topic>Polyphenols</topic><topic>Q1</topic><topic>Statistical analysis</topic><topic>Statistical models</topic><topic>Tea</topic><topic>Tea - chemistry</topic><topic>theaflavins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TU, You-Ying</creatorcontrib><creatorcontrib>XU, Xin-Qing</creatorcontrib><creatorcontrib>XIA, Hui-Long</creatorcontrib><creatorcontrib>WATANABE, Naoharu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TU, You-Ying</au><au>XU, Xin-Qing</au><au>XIA, Hui-Long</au><au>WATANABE, Naoharu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodology</atitle><jtitle>Biotechnology letters</jtitle><addtitle>Biotechnol Lett</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>27</volume><issue>4</issue><spage>269</spage><epage>274</epage><pages>269-274</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Theaflavins were synthesized from tea polyphenols extracted from green tea using an immobilized polyphenol oxidase system. To optimize the production of theaflavins, response surface methodology was applied to determine the effects of five critical variables and their mutual interactions on theaflavin biosynthesis at five levels. A total of 52 individual experiments were performed and a statistical model predicted that the highest theaflavin concentration was 0.766 mg ml(-1) at optimized conditions. Using these optimal parameters under experimental conditions in three independent replicates, the average value of the biosynthesized theaflavin concentration reached 0.75 +/- 0.017 mg ml(-1) and matched the value predicted by the model.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>15742149</pmid><doi>10.1007/s10529-004-8292-4</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2005-02, Vol.27 (4), p.269-274 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_745693719 |
| source | Springer Nature |
| subjects | Biflavonoids - chemical synthesis Biological and medical sciences Biosynthesis Biotechnology Catechin - chemical synthesis Catechol Oxidase - chemistry Combinatorial Chemistry Techniques - methods Computer Simulation Enzymes Enzymes, Immobilized - chemistry Flavonoids - chemistry Fundamental and applied biological sciences. Psychology Green tea Immobilized enzymes Mathematical models Models, Chemical Optimization Phenols - chemistry Plant Extracts - chemistry Polyphenols Q1 Statistical analysis Statistical models Tea Tea - chemistry theaflavins |
| title | Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodology |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T23%3A20%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optimization%20of%20theaflavin%20biosynthesis%20from%20tea%20polyphenols%20using%20an%20immobilized%20enzyme%20system%20and%20response%20surface%20methodology&rft.jtitle=Biotechnology%20letters&rft.au=TU,%20You-Ying&rft.date=2005-02-01&rft.volume=27&rft.issue=4&rft.spage=269&rft.epage=274&rft.pages=269-274&rft.issn=0141-5492&rft.eissn=1573-6776&rft.coden=BILED3&rft_id=info:doi/10.1007/s10529-004-8292-4&rft_dat=%3Cproquest_cross%3E6429864%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c491t-7135f652c2d78c35fcf463c06c89a9d39260e339fd690aa22f429c962a16aaff3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=734864355&rft_id=info:pmid/15742149&rfr_iscdi=true |