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Mixed Microtubules Steer Dynein-Driven Cargo Transport into Dendrites
To establish and maintain their polarized morphology, neurons employ active transport driven by molecular motors to sort cargo between axons and dendrites. However, the basic traffic rules governing polarized transport on neuronal microtubule arrays are unclear. Here we show that the microtubule min...
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Published in: | Current biology 2010-02, Vol.20 (4), p.290-299 |
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description | To establish and maintain their polarized morphology, neurons employ active transport driven by molecular motors to sort cargo between axons and dendrites. However, the basic traffic rules governing polarized transport on neuronal microtubule arrays are unclear.
Here we show that the microtubule minus-end-directed motor dynein is required for the polarized targeting of dendrite-specific cargo, such as AMPA receptors. To directly examine how dynein motors contribute to polarized dendritic transport, we established a trafficking assay in hippocampal neurons to selectively probe specific motor protein activity. This revealed that, unlike kinesins, dynein motors drive cargo selectively into dendrites, governed by their mixed microtubule array. Moreover, axon-specific cargos, such as presynaptic vesicle protein synaptophysin, are redirected to dendrites by coupling to dynein motors. Quantitative modeling demonstrated that bidirectional dynein-driven transport on mixed microtubules provides an efficient mechanism to establish a stable density of continuously renewing vesicles in dendrites.
These results demonstrate a powerful approach to study specific motor protein activity inside living cells and imply a key role for dynein in dendritic transport. We propose that dynein establishes the initial sorting of dendritic cargo and additional motor proteins assist in subsequent delivery.
[Display omitted]
► A new toolbox to probe specific motor proteins inside neurons is introduced ► Dynein selectively targets cargo to dendrites, whereas kinesins mainly target axons ► Dynein's dendritic dynamics is governed by the mixed orientations of microtubules ► Dynein inhibition disrupts the polarized sorting of AMPA receptors |
doi_str_mv | 10.1016/j.cub.2009.12.052 |
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Here we show that the microtubule minus-end-directed motor dynein is required for the polarized targeting of dendrite-specific cargo, such as AMPA receptors. To directly examine how dynein motors contribute to polarized dendritic transport, we established a trafficking assay in hippocampal neurons to selectively probe specific motor protein activity. This revealed that, unlike kinesins, dynein motors drive cargo selectively into dendrites, governed by their mixed microtubule array. Moreover, axon-specific cargos, such as presynaptic vesicle protein synaptophysin, are redirected to dendrites by coupling to dynein motors. Quantitative modeling demonstrated that bidirectional dynein-driven transport on mixed microtubules provides an efficient mechanism to establish a stable density of continuously renewing vesicles in dendrites.
These results demonstrate a powerful approach to study specific motor protein activity inside living cells and imply a key role for dynein in dendritic transport. We propose that dynein establishes the initial sorting of dendritic cargo and additional motor proteins assist in subsequent delivery.
[Display omitted]
► A new toolbox to probe specific motor proteins inside neurons is introduced ► Dynein selectively targets cargo to dendrites, whereas kinesins mainly target axons ► Dynein's dendritic dynamics is governed by the mixed orientations of microtubules ► Dynein inhibition disrupts the polarized sorting of AMPA receptors</description><identifier>ISSN: 0960-9822</identifier><identifier>EISSN: 1879-0445</identifier><identifier>DOI: 10.1016/j.cub.2009.12.052</identifier><identifier>PMID: 20137950</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; Biological Transport, Active - physiology ; CELLBIO ; Cercopithecus aethiops ; COS Cells ; Dendrites - metabolism ; Dyneins - metabolism ; Hippocampus - cytology ; Immunohistochemistry ; Kinesin - metabolism ; Microtubules - metabolism ; Models, Biological ; MOLNEURO ; Peroxisomes - metabolism ; Receptors, AMPA - metabolism ; Synaptophysin - metabolism</subject><ispartof>Current biology, 2010-02, Vol.20 (4), p.290-299</ispartof><rights>2010 Elsevier Ltd</rights><rights>Copyright 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-87479d966f61278fa7003abff79b504e810d78098d25458e10e0aad3546a00383</citedby><cites>FETCH-LOGICAL-c427t-87479d966f61278fa7003abff79b504e810d78098d25458e10e0aad3546a00383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20137950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kapitein, Lukas C.</creatorcontrib><creatorcontrib>Schlager, Max A.</creatorcontrib><creatorcontrib>Kuijpers, Marijn</creatorcontrib><creatorcontrib>Wulf, Phebe S.</creatorcontrib><creatorcontrib>van Spronsen, Myrrhe</creatorcontrib><creatorcontrib>MacKintosh, Frederick C.</creatorcontrib><creatorcontrib>Hoogenraad, Casper C.</creatorcontrib><title>Mixed Microtubules Steer Dynein-Driven Cargo Transport into Dendrites</title><title>Current biology</title><addtitle>Curr Biol</addtitle><description>To establish and maintain their polarized morphology, neurons employ active transport driven by molecular motors to sort cargo between axons and dendrites. However, the basic traffic rules governing polarized transport on neuronal microtubule arrays are unclear.
Here we show that the microtubule minus-end-directed motor dynein is required for the polarized targeting of dendrite-specific cargo, such as AMPA receptors. To directly examine how dynein motors contribute to polarized dendritic transport, we established a trafficking assay in hippocampal neurons to selectively probe specific motor protein activity. This revealed that, unlike kinesins, dynein motors drive cargo selectively into dendrites, governed by their mixed microtubule array. Moreover, axon-specific cargos, such as presynaptic vesicle protein synaptophysin, are redirected to dendrites by coupling to dynein motors. Quantitative modeling demonstrated that bidirectional dynein-driven transport on mixed microtubules provides an efficient mechanism to establish a stable density of continuously renewing vesicles in dendrites.
These results demonstrate a powerful approach to study specific motor protein activity inside living cells and imply a key role for dynein in dendritic transport. We propose that dynein establishes the initial sorting of dendritic cargo and additional motor proteins assist in subsequent delivery.
[Display omitted]
► A new toolbox to probe specific motor proteins inside neurons is introduced ► Dynein selectively targets cargo to dendrites, whereas kinesins mainly target axons ► Dynein's dendritic dynamics is governed by the mixed orientations of microtubules ► Dynein inhibition disrupts the polarized sorting of AMPA receptors</description><subject>Animals</subject><subject>Biological Transport, Active - physiology</subject><subject>CELLBIO</subject><subject>Cercopithecus aethiops</subject><subject>COS Cells</subject><subject>Dendrites - metabolism</subject><subject>Dyneins - metabolism</subject><subject>Hippocampus - cytology</subject><subject>Immunohistochemistry</subject><subject>Kinesin - metabolism</subject><subject>Microtubules - metabolism</subject><subject>Models, Biological</subject><subject>MOLNEURO</subject><subject>Peroxisomes - metabolism</subject><subject>Receptors, AMPA - metabolism</subject><subject>Synaptophysin - metabolism</subject><issn>0960-9822</issn><issn>1879-0445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqF0L1OwzAUhmELgWgpXAALysaUcOzEsS0m1JYfqRUDZbac-AS5apNiJxW9e1K1MMJ0lvd8w0PINYWEAs3vlknZFQkDUAllCXB2QoZUChVDlvFTMgSVQ6wkYwNyEcISgDKp8nMyYEBToTgMyXTuvtBGc1f6pu2KboUhemsRfTTZ1ejqeOLdFutobPxHEy28qcOm8W3k6raJJlhb71oMl-SsMquAV8c7Iu-P08X4OZ69Pr2MH2ZxmTHRxlJkQlmV51VOmZCVEQCpKapKqIJDhpKCFRKUtIxnXCIFBGNsyrPc9KVMR-T2sLvxzWeHodVrF0pcrUyNTRe0yHiuBJf8_zJNKZeUQ1_SQ9kLhOCx0hvv1sbvNAW9Z9ZL3TPrPbOmTPfM_c_Ncb0r1mh_P35c--D-EGCvsXXodSgd1iVa57FstW3cH_Pf7fuLlA</recordid><startdate>20100223</startdate><enddate>20100223</enddate><creator>Kapitein, Lukas C.</creator><creator>Schlager, Max A.</creator><creator>Kuijpers, Marijn</creator><creator>Wulf, Phebe S.</creator><creator>van Spronsen, Myrrhe</creator><creator>MacKintosh, Frederick C.</creator><creator>Hoogenraad, Casper C.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20100223</creationdate><title>Mixed Microtubules Steer Dynein-Driven Cargo Transport into Dendrites</title><author>Kapitein, Lukas C. ; Schlager, Max A. ; Kuijpers, Marijn ; Wulf, Phebe S. ; van Spronsen, Myrrhe ; MacKintosh, Frederick C. ; Hoogenraad, Casper C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-87479d966f61278fa7003abff79b504e810d78098d25458e10e0aad3546a00383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological Transport, Active - physiology</topic><topic>CELLBIO</topic><topic>Cercopithecus aethiops</topic><topic>COS Cells</topic><topic>Dendrites - metabolism</topic><topic>Dyneins - metabolism</topic><topic>Hippocampus - cytology</topic><topic>Immunohistochemistry</topic><topic>Kinesin - metabolism</topic><topic>Microtubules - metabolism</topic><topic>Models, Biological</topic><topic>MOLNEURO</topic><topic>Peroxisomes - metabolism</topic><topic>Receptors, AMPA - metabolism</topic><topic>Synaptophysin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kapitein, Lukas C.</creatorcontrib><creatorcontrib>Schlager, Max A.</creatorcontrib><creatorcontrib>Kuijpers, Marijn</creatorcontrib><creatorcontrib>Wulf, Phebe S.</creatorcontrib><creatorcontrib>van Spronsen, Myrrhe</creatorcontrib><creatorcontrib>MacKintosh, Frederick C.</creatorcontrib><creatorcontrib>Hoogenraad, Casper C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Current biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kapitein, Lukas C.</au><au>Schlager, Max A.</au><au>Kuijpers, Marijn</au><au>Wulf, Phebe S.</au><au>van Spronsen, Myrrhe</au><au>MacKintosh, Frederick C.</au><au>Hoogenraad, Casper C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mixed Microtubules Steer Dynein-Driven Cargo Transport into Dendrites</atitle><jtitle>Current biology</jtitle><addtitle>Curr Biol</addtitle><date>2010-02-23</date><risdate>2010</risdate><volume>20</volume><issue>4</issue><spage>290</spage><epage>299</epage><pages>290-299</pages><issn>0960-9822</issn><eissn>1879-0445</eissn><abstract>To establish and maintain their polarized morphology, neurons employ active transport driven by molecular motors to sort cargo between axons and dendrites. However, the basic traffic rules governing polarized transport on neuronal microtubule arrays are unclear.
Here we show that the microtubule minus-end-directed motor dynein is required for the polarized targeting of dendrite-specific cargo, such as AMPA receptors. To directly examine how dynein motors contribute to polarized dendritic transport, we established a trafficking assay in hippocampal neurons to selectively probe specific motor protein activity. This revealed that, unlike kinesins, dynein motors drive cargo selectively into dendrites, governed by their mixed microtubule array. Moreover, axon-specific cargos, such as presynaptic vesicle protein synaptophysin, are redirected to dendrites by coupling to dynein motors. Quantitative modeling demonstrated that bidirectional dynein-driven transport on mixed microtubules provides an efficient mechanism to establish a stable density of continuously renewing vesicles in dendrites.
These results demonstrate a powerful approach to study specific motor protein activity inside living cells and imply a key role for dynein in dendritic transport. We propose that dynein establishes the initial sorting of dendritic cargo and additional motor proteins assist in subsequent delivery.
[Display omitted]
► A new toolbox to probe specific motor proteins inside neurons is introduced ► Dynein selectively targets cargo to dendrites, whereas kinesins mainly target axons ► Dynein's dendritic dynamics is governed by the mixed orientations of microtubules ► Dynein inhibition disrupts the polarized sorting of AMPA receptors</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>20137950</pmid><doi>10.1016/j.cub.2009.12.052</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biological Transport, Active - physiology CELLBIO Cercopithecus aethiops COS Cells Dendrites - metabolism Dyneins - metabolism Hippocampus - cytology Immunohistochemistry Kinesin - metabolism Microtubules - metabolism Models, Biological MOLNEURO Peroxisomes - metabolism Receptors, AMPA - metabolism Synaptophysin - metabolism |
title | Mixed Microtubules Steer Dynein-Driven Cargo Transport into Dendrites |
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