High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak

Summary Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are una...

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Bibliographic Details
Published in:The Journal of hospital infection 2010-05, Vol.75 (1), p.37-41
Main Authors: Lewis, T, Loman, N.J, Bingle, L, Jumaa, P, Weinstock, G.M, Mortiboy, D, Pallen, M.J
Format: Article
Language:English
Subjects:
454 pyrosequencing
Acinetobacter baumannii
Acinetobacter baumannii - classification
Acinetobacter baumannii - genetics
Acinetobacter baumannii - isolation & purification
Acinetobacter Infections - epidemiology
Acinetobacter Infections - microbiology
Bacterial Typing Techniques
Biological and medical sciences
Cross Infection - epidemiology
Cross Infection - microbiology
Disease Outbreaks
Disease transmission
Drug resistance
Drug Resistance, Multiple, Bacterial
Epidemiology
General aspects
Genome, Bacterial
Genotype
Hospitals
Human infectious diseases. Experimental studies and models
Humans
Infectious Disease
Infectious diseases
Male
Medical sciences
Multidrug resistance
Polymorphism, Single Nucleotide
Sequence Analysis, DNA
Single nucleotide polymorphism
Typing
United Kingdom - epidemiology
Whole-genome sequencing
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Summary:Summary Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.
ISSN:0195-6701
1532-2939
DOI:10.1016/j.jhin.2010.01.012