Upregulation of Monocyte Tissue Factor Activity Is Significantly Associated With Low-Grade Chronic Inflammation and Insulin Resistance in Patients With Metabolic Syndrome

Background: The metabolic syndrome (MetS) is a cluster of cardiovascular risk factors closely linked to inflammation and insulin resistance (IR). Tissue factor (TF) is an initiator of the extrinsic coagulation cascade and is expressed on peripheral blood monocytes and macrophages in atherosclerotic...

Full description

Saved in:
Bibliographic Details
Published in:Circulation Journal 2010, Vol.74(3), pp.572-577
Main Authors: Nakagomi, Akihiro, Sasaki, Mihoko, Ishikawa, Youhei, Morikawa, Masako, Shibui, Toshiyuki, Kusama, Yoshiki, Atarashi, Hirotsugu, Mizuno, Kyoichi
Format: Article
Language:English
Subjects:
Aged
C-Reactive Protein - metabolism
Chronic Disease
Disease Progression
Female
Homeostasis - immunology
Humans
In Vitro Techniques
Inflammation
Inflammation - epidemiology
Inflammation - immunology
Inflammation - metabolism
Insulin resistance
Insulin Resistance - immunology
Lipopolysaccharides - blood
Lipopolysaccharides - pharmacology
Male
Metabolic syndrome
Metabolic Syndrome - epidemiology
Metabolic Syndrome - immunology
Metabolic Syndrome - metabolism
Middle Aged
Monocytes - drug effects
Monocytes - immunology
Monocytes - metabolism
Risk Factors
Thromboplastin - immunology
Thromboplastin - metabolism
Tissue factor
Up-Regulation - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: The metabolic syndrome (MetS) is a cluster of cardiovascular risk factors closely linked to inflammation and insulin resistance (IR). Tissue factor (TF) is an initiator of the extrinsic coagulation cascade and is expressed on peripheral blood monocytes and macrophages in atherosclerotic plaques. Monocytes are the principle cells capable of TF synthesis. Therefore, TF plays an important role in both thrombosis and atherosclerosis. Elevated levels of lipopolysaccharide (LPS), a strong stimulator of TF, have been observed in patients with MetS. No study has investigated the relationship between monocyte TF activity and inflammation, and IR in MetS. Methods and Results: Peripheral blood mononuclear cells (PBMCs) were collected from 40 normal subjects and 77 patients with MetS. Mononuclear cell TF procoagulant activity (MPCA) was measured with and without 100 pg/ml LPS stimulation using a 1-stage clotting assay and expressed as the mean ± SD (mU TF/106 PBMCs). MPCA in MetS was significantly greater than in normal subjects (without LPS: 88.0±74.8 vs 52.6±9.8 mU TF/106 PBMCs, P
ISSN:1346-9843
1347-4820
DOI:10.1253/circj.CJ-09-0835