Estimation of ochratoxin A in some Turkish populations: An analysis in urine as a simple, sensitive and reliable biomarker

• Published in: Food and chemical toxicology 2010-03, Vol.48 (3), p.877-882
• Main Authors: Akdemir, Cigdem, Ulker, Ozge Cemiloglu, Basaran, Alaattin, Ozkaya, Sennur, Karakaya, Asuman
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Alcohol Drinking - metabolism; Anthropometry; Balkan Endemic Nephropathy; BEN; Biological and medical sciences; Biomarkers; Carcinogens - metabolism; Chromatography, High Pressure Liquid; Coffee; Creatinine - blood; CYP450; cytochrome P450; Diet; EFSA; European Food Safety Authority; Female; high performance liquid chromatography; HPLC; Human exposure; Humans; IAC; IARC; Immunoaffinity column clean-up; immunoaffinity columns; Immunochemistry; JECFA; Life Style; limit of detection; limit of quantification; LOAEL; LOD; LOQ; lowest observed adverse effect level; Male; Medical sciences; Middle Aged; ochratoxin A; Ochratoxins - urine; OTA; Plant poisons toxicology; Reproducibility of Results; SCF; Scientific Committee on Food; Sex Factors; Smoking - adverse effects; Surveys and Questionnaires; The International Agency for Research on Cancer; The Joint FAO/WHO Expert Committee on Food Additives; tolerable weekly intake; Toxicology; Turkey; TWI; Urine; WHO; World Health Organization; Young Adult
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2009.12.026

In this study, ochratoxin A (OTA) occurrence and level in human urine samples, collected from four different regions of Turkey was analyzed by NaHCO3 dilution, immunoaffinity column clean-up and high-performance liquid chromatography with fluorescent detection. For the repeatability of the method, RSD (%) values were found between 3.83 and 8.86, for the accuracy, the recovery values were found between 85.7% and 110.5% and limit of detection and limit of quantification of the method were measured as 0.006 and 0.018ngmL−1 respectively. For the analysis, first morning urine samples were collected and the results were adjusted with creatinine levels. From the total collected samples of 233 larger amounts of 83% was contaminated with OTA. Among the calculated to be OTA levels, positive sample rate, average OTA amount and the highest contamination was found in Ankara. (Positive sample rate; 90.1%, average OTA concentration; 14.34ngg−1 creatinine and highest OTA value; 75.60ngg−1 creatinine). In order to define the exposure profile of OTA in human a questionnaire was conducted among the voluntaries as well. But related to the gender, age, dietary habits, coffee consumption, smoking and alcohol habits of the volunteers, no correlation was found with the OTA exposure.