study of adaptation mechanisms of Yarrowia lipolytica yeast to alkaline conditions by means of proteomics

Using proteomic technologies—two-dimensional electrophoresis in denaturing conditions in combination with mass spectroscopy of MALDI-TOF proteins—we demonstrated, for the first time, that the most noticeable alteration of protein composition of a Yarrowia lipolytica cell during adaptation to alkalin...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2010-05, Vol.46 (3), p.307-312
Main Authors: Guseva, M. A, Epova, E. Yu, Kovalev, L. I, Shevelev, A. B
Format: Article
Language:English
Subjects:
Alkalinity
Biochemistry
Biomedical and Life Sciences
Cellular biology
Life Sciences
Mass spectroscopy
Medical Microbiology
Microbiology
Proteomics
Yarrowia lipolytica
Yeasts
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Summary:Using proteomic technologies—two-dimensional electrophoresis in denaturing conditions in combination with mass spectroscopy of MALDI-TOF proteins—we demonstrated, for the first time, that the most noticeable alteration of protein composition of a Yarrowia lipolytica cell during adaptation to alkaline conditions was an increase of mitochondrial proteins relatively to proteins of cytoplasm.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683810030105