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Neutralizing single-chain antibodies against the tick-borne encephalitis virus

A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in Escherichia coli cells of the...

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2009-07, Vol.35 (4), p.474-481
Main Authors: Levanov, L. N., Matveev, L. E., Yun, T. E., Goncharova, E. P., Lebedev, L. R., Shvalov, A. N., Ryzhykov, A. B., Baikov, I. K., Matveeva, V. A., Rikhter, V. A., Tikunova, N. V.
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Language:English
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Summary:A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in Escherichia coli cells of the scl3D6 single-chain antibody against the TBE virus. The produced antibodies could bind to the TBE virus, strain 205, and the TBE virus recombinant E protein. The affinity constant of purified scl3D6 was (3.0 ± 0.2) × 10 7 M −1 for the equilibrium state and (2.8 ± 0.3) × 10 7 M −1 in the case of antigen-antibody formation on the surface. The obtained single-chain antibody could inhibit the infection potency of the TBE virus on a monolayer of eukaryotic cells. The calculated IC 50 value for scl3D6 was 16.7 μg/ml.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162009040098