The RCAF complex mediates chromatin assembly during DNA replication and repair

Chromatin assembly is a fundamental biological process that is essential for the replication and maintenance of the eukaryotic genome. In dividing cells, newly synthesized DNA is rapidly assembled into chromatin by the deposition of a tetramer of the histone proteins H3 and H4, followed by the depos...

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Bibliographic Details
Published in:Nature (London) 1999-12, Vol.402 (6761), p.555-560
Main Authors: Tyler, Jessica K, Adams, Christopher R, Chen, Shaw-Ree, Kobayashi, Ryuji, Kamakaka, Rohinton T, Kadonaga, James T
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
ASF1 gene
Biological and medical sciences
Cell Cycle Proteins - genetics
Cell Cycle Proteins - isolation & purification
Cell Cycle Proteins - physiology
Cellular biology
Chromatin - metabolism
Cloning
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA Repair
DNA Replication
Drosophila
Drosophila - genetics
Drosophila - physiology
Drosophila Proteins
Fundamental and applied biological sciences. Psychology
Gene Silencing
Genetics
HeLa Cells
histone H3
histone H4
Humanities and Social Sciences
Humans
letter
Molecular and cellular biology
Molecular Chaperones - physiology
Molecular genetics
Molecular Sequence Data
multidisciplinary
Proteins
RCAF protein
replication-coupling assembly factor
Saccharomyces cerevisiae
Science
Science (multidisciplinary)
Translation. Translation factors. Protein processing
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Summary:Chromatin assembly is a fundamental biological process that is essential for the replication and maintenance of the eukaryotic genome. In dividing cells, newly synthesized DNA is rapidly assembled into chromatin by the deposition of a tetramer of the histone proteins H3 and H4, followed by the deposition of two dimers of histones H2A and H2B to complete the nucleosome-the fundamental repeating unit of chromatin. Here we describe the identification, purification, cloning, and characterization of replication-coupling assembly factor (RCAF), a novel protein complex that facilitates the assembly of nucleosomes onto newly replicated DNA in vitro. RCAF comprises the Drosophila homologue of anti-silencing function 1 protein ASF1 and histones H3 and H4. The specific acetylation pattern of H3 and H4 in RCAF is identical to that of newly synthesized histones. Genetic analyses in Saccharomyces cerevisiae demonstrate that ASF1 is essential for normal cell cycle progression, and suggest that RCAF mediates chromatin assembly after DNA replication and the repair of double-strand DNA damage in vivo.
ISSN:0028-0836
1476-4687
DOI:10.1038/990147