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Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs
Lactic acid bacteria isolated from Irish pork and beef abattoirs were analysed for their susceptibility to antimicrobials. Thirty-seven isolates (12 enterococci, 10 lactobacilli, 8 streptococci, 3 lactococci, 2 Leuconostoc, and 2 pediococci) were examined for phenotypic resistance using the E-test a...
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Published in: | Research in microbiology 2010-03, Vol.161 (2), p.127-135 |
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description | Lactic acid bacteria isolated from Irish pork and beef abattoirs were analysed for their susceptibility to antimicrobials. Thirty-seven isolates (12 enterococci, 10 lactobacilli, 8 streptococci, 3 lactococci, 2
Leuconostoc, and 2 pediococci) were examined for phenotypic resistance using the E-test and their minimum inhibitory concentration to a panel of six antibiotics (ampicillin, chloramphenicol, erythromycin, streptomycin, tetracycline, and vancomycin) was recorded. The corresponding genetic determinants responsible were characterised by PCR. Also, the transferability of these resistance markers was assessed in filter mating assays. Of the 37 isolates, 33 were found to be resistant to one or more antibiotics. All strains were susceptible to ampicillin and chloramphenicol. The
erm(B) and
msrA/B genes were detected among the 11 erythromycin-resistant strains of enterococci, lactobacilli, and streptococci. Two tetracycline-resistant strains,
Lactobacillus plantarum and
Leuconostoc mesenteroides spp., contained
tet(M) and
tet(S) genes respectively. Intrinsic streptomycin resistance was observed in lactobacilli, streptococci, lactococci and
Leuconostoc species; none of the common genetic determinants (
strA,
strB,
aadA,
aadE) were identified. Four of 10 strains of
Enterococcus faecium were resistant to vancomycin; however, no corresponding genetic determinants for this phenotype were identified.
Enterococcus faecalis strains were susceptible to vancomycin.
L. plantarum,
L. mesenteroides and
Pediococcus pentosaceus were intrinsically resistant to vancomycin. Transfer of antibiotic resistance determinants was demonstrated in one strain, wherein the
tet(M) gene of
L. plantarum (23) isolated from a pork abattoir was transferred to
Lactococcus lactis BU-2-60 and to
E. faecalis JH2-2. This study identified the presence of antibiotic resistance markers in Irish meat isolates and, in one example, resistance was conjugally transferred to other LAB strains. |
doi_str_mv | 10.1016/j.resmic.2009.12.010 |
format | article |
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Leuconostoc, and 2 pediococci) were examined for phenotypic resistance using the E-test and their minimum inhibitory concentration to a panel of six antibiotics (ampicillin, chloramphenicol, erythromycin, streptomycin, tetracycline, and vancomycin) was recorded. The corresponding genetic determinants responsible were characterised by PCR. Also, the transferability of these resistance markers was assessed in filter mating assays. Of the 37 isolates, 33 were found to be resistant to one or more antibiotics. All strains were susceptible to ampicillin and chloramphenicol. The
erm(B) and
msrA/B genes were detected among the 11 erythromycin-resistant strains of enterococci, lactobacilli, and streptococci. Two tetracycline-resistant strains,
Lactobacillus plantarum and
Leuconostoc mesenteroides spp., contained
tet(M) and
tet(S) genes respectively. Intrinsic streptomycin resistance was observed in lactobacilli, streptococci, lactococci and
Leuconostoc species; none of the common genetic determinants (
strA,
strB,
aadA,
aadE) were identified. Four of 10 strains of
Enterococcus faecium were resistant to vancomycin; however, no corresponding genetic determinants for this phenotype were identified.
Enterococcus faecalis strains were susceptible to vancomycin.
L. plantarum,
L. mesenteroides and
Pediococcus pentosaceus were intrinsically resistant to vancomycin. Transfer of antibiotic resistance determinants was demonstrated in one strain, wherein the
tet(M) gene of
L. plantarum (23) isolated from a pork abattoir was transferred to
Lactococcus lactis BU-2-60 and to
E. faecalis JH2-2. This study identified the presence of antibiotic resistance markers in Irish meat isolates and, in one example, resistance was conjugally transferred to other LAB strains.</description><identifier>ISSN: 0923-2508</identifier><identifier>EISSN: 1769-7123</identifier><identifier>DOI: 10.1016/j.resmic.2009.12.010</identifier><identifier>PMID: 20074643</identifier><language>eng</language><publisher>Issy-les-Moulineaux: Elsevier SAS</publisher><subject>Abattoirs ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Biological and medical sciences ; Conjugation, Genetic ; DNA, Bacterial - genetics ; Drug Resistance, Bacterial ; Enterococcus faecalis ; Enterococcus faecium ; Fundamental and applied biological sciences. Psychology ; Gene transfer ; Gene Transfer, Horizontal ; Genes, Bacterial ; Gram-Positive Bacteria - drug effects ; Gram-Positive Bacteria - genetics ; Gram-Positive Bacteria - isolation & purification ; Gram-Positive Bacteria - metabolism ; Ireland ; Irish meat plants ; Lactic Acid - metabolism ; Lactic acid bacteria ; Lactobacillus ; Lactobacillus plantarum ; Lactococcus lactis ; Leuconostoc ; Leuconostoc mesenteroides ; Microbial Sensitivity Tests ; Microbiology ; Pediococcus pentosaceus ; Polymerase Chain Reaction</subject><ispartof>Research in microbiology, 2010-03, Vol.161 (2), p.127-135</ispartof><rights>2010 Elsevier Masson SAS</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-f702f0a91c232bf58577bc44e14070672bed8623858837bf7afa020a8dbbcae63</citedby><cites>FETCH-LOGICAL-c423t-f702f0a91c232bf58577bc44e14070672bed8623858837bf7afa020a8dbbcae63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22758616$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20074643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toomey, Niamh</creatorcontrib><creatorcontrib>Bolton, Declan</creatorcontrib><creatorcontrib>Fanning, Séamus</creatorcontrib><title>Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs</title><title>Research in microbiology</title><addtitle>Res Microbiol</addtitle><description>Lactic acid bacteria isolated from Irish pork and beef abattoirs were analysed for their susceptibility to antimicrobials. Thirty-seven isolates (12 enterococci, 10 lactobacilli, 8 streptococci, 3 lactococci, 2
Leuconostoc, and 2 pediococci) were examined for phenotypic resistance using the E-test and their minimum inhibitory concentration to a panel of six antibiotics (ampicillin, chloramphenicol, erythromycin, streptomycin, tetracycline, and vancomycin) was recorded. The corresponding genetic determinants responsible were characterised by PCR. Also, the transferability of these resistance markers was assessed in filter mating assays. Of the 37 isolates, 33 were found to be resistant to one or more antibiotics. All strains were susceptible to ampicillin and chloramphenicol. The
erm(B) and
msrA/B genes were detected among the 11 erythromycin-resistant strains of enterococci, lactobacilli, and streptococci. Two tetracycline-resistant strains,
Lactobacillus plantarum and
Leuconostoc mesenteroides spp., contained
tet(M) and
tet(S) genes respectively. Intrinsic streptomycin resistance was observed in lactobacilli, streptococci, lactococci and
Leuconostoc species; none of the common genetic determinants (
strA,
strB,
aadA,
aadE) were identified. Four of 10 strains of
Enterococcus faecium were resistant to vancomycin; however, no corresponding genetic determinants for this phenotype were identified.
Enterococcus faecalis strains were susceptible to vancomycin.
L. plantarum,
L. mesenteroides and
Pediococcus pentosaceus were intrinsically resistant to vancomycin. Transfer of antibiotic resistance determinants was demonstrated in one strain, wherein the
tet(M) gene of
L. plantarum (23) isolated from a pork abattoir was transferred to
Lactococcus lactis BU-2-60 and to
E. faecalis JH2-2. This study identified the presence of antibiotic resistance markers in Irish meat isolates and, in one example, resistance was conjugally transferred to other LAB strains.</description><subject>Abattoirs</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Biological and medical sciences</subject><subject>Conjugation, Genetic</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Bacterial</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecium</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene transfer</subject><subject>Gene Transfer, Horizontal</subject><subject>Genes, Bacterial</subject><subject>Gram-Positive Bacteria - drug effects</subject><subject>Gram-Positive Bacteria - genetics</subject><subject>Gram-Positive Bacteria - isolation & purification</subject><subject>Gram-Positive Bacteria - metabolism</subject><subject>Ireland</subject><subject>Irish meat plants</subject><subject>Lactic Acid - metabolism</subject><subject>Lactic acid bacteria</subject><subject>Lactobacillus</subject><subject>Lactobacillus plantarum</subject><subject>Lactococcus lactis</subject><subject>Leuconostoc</subject><subject>Leuconostoc mesenteroides</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Pediococcus pentosaceus</subject><subject>Polymerase Chain Reaction</subject><issn>0923-2508</issn><issn>1769-7123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1DAUhS0EotPCGyDkDeoqwT9JnGyQ0KgtlSqxgbV17VxTD0k82J5KfQTeGk8zwA5WluzvnON7DyFvOKs54937XR0xzd7WgrGh5qJmnD0jG666oVJcyOdkwwYhK9Gy_oycp7RjjLdKNS_JWZGopmvkhvzc3kMEmzH6BNmHhcIy0hxhSQ4jGD_5_EiDK9fZGx-yt7Tk-pRhsUi_4YKJuhhmOhWX8gjWj9SsjkB9ChNkHFfktoTc032I359SDGLxNZBz8DG9Ii8cTAlfn84L8vX66sv2U3X3-eZ2-_Guso2QuXKKCcdg4FZIYVzbl5GMbRrkDVOsU8Lg2HdC9m3fS2WcAgdMMOhHYyxgJy_I5eq7j-HHAVPWs08WpwkWDIekVdOW9fVD-39SSt4OnRwK2aykjSGliE7vo58hPmrO9LEtvdNrW_rYluZCl7aK7O0p4GBmHP-IftdTgHcnAJKFyZVarE9_OaHavuPHmT6sHJbFPXiMOlmPpaDRR7RZj8H_-ye_AM__tyU</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Toomey, Niamh</creator><creator>Bolton, Declan</creator><creator>Fanning, Séamus</creator><general>Elsevier SAS</general><general>Elsevier Masson</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100301</creationdate><title>Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs</title><author>Toomey, Niamh ; Bolton, Declan ; Fanning, Séamus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-f702f0a91c232bf58577bc44e14070672bed8623858837bf7afa020a8dbbcae63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Abattoirs</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance</topic><topic>Biological and medical sciences</topic><topic>Conjugation, Genetic</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Bacterial</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecium</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene transfer</topic><topic>Gene Transfer, Horizontal</topic><topic>Genes, Bacterial</topic><topic>Gram-Positive Bacteria - drug effects</topic><topic>Gram-Positive Bacteria - genetics</topic><topic>Gram-Positive Bacteria - isolation & purification</topic><topic>Gram-Positive Bacteria - metabolism</topic><topic>Ireland</topic><topic>Irish meat plants</topic><topic>Lactic Acid - metabolism</topic><topic>Lactic acid bacteria</topic><topic>Lactobacillus</topic><topic>Lactobacillus plantarum</topic><topic>Lactococcus lactis</topic><topic>Leuconostoc</topic><topic>Leuconostoc mesenteroides</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Pediococcus pentosaceus</topic><topic>Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toomey, Niamh</creatorcontrib><creatorcontrib>Bolton, Declan</creatorcontrib><creatorcontrib>Fanning, Séamus</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Research in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toomey, Niamh</au><au>Bolton, Declan</au><au>Fanning, Séamus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs</atitle><jtitle>Research in microbiology</jtitle><addtitle>Res Microbiol</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>161</volume><issue>2</issue><spage>127</spage><epage>135</epage><pages>127-135</pages><issn>0923-2508</issn><eissn>1769-7123</eissn><abstract>Lactic acid bacteria isolated from Irish pork and beef abattoirs were analysed for their susceptibility to antimicrobials. Thirty-seven isolates (12 enterococci, 10 lactobacilli, 8 streptococci, 3 lactococci, 2
Leuconostoc, and 2 pediococci) were examined for phenotypic resistance using the E-test and their minimum inhibitory concentration to a panel of six antibiotics (ampicillin, chloramphenicol, erythromycin, streptomycin, tetracycline, and vancomycin) was recorded. The corresponding genetic determinants responsible were characterised by PCR. Also, the transferability of these resistance markers was assessed in filter mating assays. Of the 37 isolates, 33 were found to be resistant to one or more antibiotics. All strains were susceptible to ampicillin and chloramphenicol. The
erm(B) and
msrA/B genes were detected among the 11 erythromycin-resistant strains of enterococci, lactobacilli, and streptococci. Two tetracycline-resistant strains,
Lactobacillus plantarum and
Leuconostoc mesenteroides spp., contained
tet(M) and
tet(S) genes respectively. Intrinsic streptomycin resistance was observed in lactobacilli, streptococci, lactococci and
Leuconostoc species; none of the common genetic determinants (
strA,
strB,
aadA,
aadE) were identified. Four of 10 strains of
Enterococcus faecium were resistant to vancomycin; however, no corresponding genetic determinants for this phenotype were identified.
Enterococcus faecalis strains were susceptible to vancomycin.
L. plantarum,
L. mesenteroides and
Pediococcus pentosaceus were intrinsically resistant to vancomycin. Transfer of antibiotic resistance determinants was demonstrated in one strain, wherein the
tet(M) gene of
L. plantarum (23) isolated from a pork abattoir was transferred to
Lactococcus lactis BU-2-60 and to
E. faecalis JH2-2. This study identified the presence of antibiotic resistance markers in Irish meat isolates and, in one example, resistance was conjugally transferred to other LAB strains.</abstract><cop>Issy-les-Moulineaux</cop><pub>Elsevier SAS</pub><pmid>20074643</pmid><doi>10.1016/j.resmic.2009.12.010</doi><tpages>9</tpages></addata></record> |
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subjects | Abattoirs Anti-Bacterial Agents - pharmacology Antibiotic resistance Biological and medical sciences Conjugation, Genetic DNA, Bacterial - genetics Drug Resistance, Bacterial Enterococcus faecalis Enterococcus faecium Fundamental and applied biological sciences. Psychology Gene transfer Gene Transfer, Horizontal Genes, Bacterial Gram-Positive Bacteria - drug effects Gram-Positive Bacteria - genetics Gram-Positive Bacteria - isolation & purification Gram-Positive Bacteria - metabolism Ireland Irish meat plants Lactic Acid - metabolism Lactic acid bacteria Lactobacillus Lactobacillus plantarum Lactococcus lactis Leuconostoc Leuconostoc mesenteroides Microbial Sensitivity Tests Microbiology Pediococcus pentosaceus Polymerase Chain Reaction |
title | Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs |
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