Integrin-dependent cell behavior on ECM peptide-conjugated chitosan membranes

• Published in: Biopolymers 2007, Vol.88 (2), p.122-130
• Main Authors: Mochizuki, Mayumi, Yamagata, Natsumi, Philp, Deborah, Hozumi, Kentaro, Watanabe, Tadashi, Kikkawa, Yamato, Kadoya, Yuichi, Kleinman, Hynda K., Nomizu, Motoyoshi
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Amino Acid Sequence; Animals; cell adhesion; Cell Adhesion - drug effects; Cell Adhesion - physiology; Cells, Cultured; chitosan; Chitosan - chemistry; Edetic Acid - pharmacology; extracellular matrix (ECM); Extracellular Matrix Proteins - chemistry; Heparin - pharmacology; Humans; integrin; Integrins - physiology; laminin; Membranes, Artificial; Molecular Sequence Data; Neurites - physiology; Neurites - ultrastructure; PC12 Cells; peptide; Peptides - chemistry; Rats
• ISSN: 0006-3525; 1097-0282
• DOI: 10.1002/bip.20684

Extracellular matrix (ECM) plays an important role in tissue regeneration by promoting cell adhesion, migration, proliferation, and differentiation. ECM mimetics are of importance for tissue engineering because of their functions as scaffolds for cells. Previously, we developed bioactive laminin‐derived peptide‐conjugated chitosan membranes and demonstrated their cell‐ and peptide‐type specific functions. Here, we conjugated twelve integrin‐binding peptides derived from ECM proteins onto chitosan membranes and examined biological activity. Seven peptide–chitosan membranes promoted human foreskin fibroblast attachment. Additionally, FIB1 (YAVTGRGDSPAS; from fibronectin), A99 (AGTFALRGDNPQG; from laminin α1 chain), EF1zz (ATLQLQEGRLHFXFDLGKGR, X = Nle; from laminin α1 chain), and 531 (GEFYFDLRLKGDKY; from collagen α1 (IV) chain) conjugated chitosan membranes promoted integrin‐dependent cell adhesion. Various integrins, including αv, β1, and β3, were involved in the cell adhesion to the peptide–chitosan membranes. Further, only the FIB1‐ and A99‐chitosan membranes promoted neurite outgrowth with PC12 rat pheochromocytoma cells. These data demonstrate that peptide–chitosan membranes can regulate specific integrin‐mediated cell responses and are useful constructs as ECM mimetics. © 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 88: 122–130, 2007. This article was originally published online as an accepted preprint. The ‘Published Online’ date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [e-mail]