Ferritin Heavy Chain (FHC) is Up-regulated in Papillomavirus-Associated Urothelial Tumours of the Urinary Bladder in Cattle

• Published in: Journal of comparative pathology 2010, Vol.142 (1), p.9-18
• Main Authors: Roperto, S., Borzacchiello, G., Brun, R., Costanzo, F., Faniello, M.C., Raso, C., Rosati, A., Russo, V., Leonardi, L., Saracino, D., Turco, M.C., Urraro, C., Roperto, F.
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Base Sequence; bladder diseases; Blotting, Western; Bovine papillomavirus; Bovine papillomavirus 1; Cattle; Cattle Diseases - metabolism; Cattle Diseases - virology; Confocal microscopy; Electrophoretic Mobility Shift Assay; epithelium; Ferritin; ferritin heavy chain; Ferritins - genetics; Ferritins - metabolism; Fluorescent Antibody Technique; gene expression regulation; gene overexpression; gene up-regulation; Grazing; Humans; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Microscopy, Confocal; Molecular Sequence Data; Mountains; neoplasms; NF-B protein; NF-kappa B - biosynthesis; nuclear factor-kappaB; Oligonucleotides; oncogenic viruses; papillomavirus; Papillomavirus Infections - complications; Papillomavirus Infections - metabolism; Papillomavirus Infections - veterinary; Pasture; Polymerase chain reaction; protein subunits; Reverse Transcriptase Polymerase Chain Reaction; RNA-directed DNA polymerase; sequence analysis; Sequence Homology, Nucleic Acid; Transcription factors; Tumors; Up-Regulation; Urinary bladder; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - veterinary; Urinary Bladder Neoplasms - virology; urothelial tumors; urothelial tumour; Western blotting
• ISSN: 0021-9975; 1532-3129
• DOI: 10.1016/j.jcpa.2009.05.009

The up-regulation of ferritin heavy chain (FHC) is reported in six papillary and in four invasive urothelial tumours of the urinary bladder of cattle grazing on mountain pastures rich in bracken fern. All tumours contained sequence of bovine papillomavirus type-2 (BPV-2) as determined by polymerase chain reaction (PCR) analyses and validated by direct sequencing of the amplified products. The oncoprotein E5 was also detected in these tumours by immunoprecipitation and by immunofluorescence and laser scanning confocal microscopy. Expression of FHC was evaluated by western blot analysis, reverse transcriptase (RT) PCR, real-time RT-PCR and immunohistochemistry. The oligonucleotide sequence of the bovine ferritin amplicons was identical to that of human ferritin. Nuclear overexpression of p65, an important component of nuclear factor κB (NF-κB) transcription factors, was also observed. These findings suggest that FHC up-regulation may be mediated by activation of NF-κB and that in turn this may be related to the resistance of bovine papillomavirus type-2 (BPV-2) infected urothelial cells to apoptosis.