Chromosome variability of human multipotent mesenchymal stromal cells

We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passag...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2007-01, Vol.143 (1), p.122-126
Main Authors: Bochkov, N P, Voronina, E S, Kosyakova, N V, Liehr, T, Rzhaninova, A A, Katosova, L D, Platonova, V I, Gol'dshtein, D V
Format: Article
Language:English
Subjects:
Adult
Cell Culture Techniques
Cells, Cultured
Chromosome Aberrations
Chromosomes, Human
Cytogenetic Analysis
Humans
Karyotyping
Mesenchymal Stem Cells - cytology
Middle Aged
Multipotent Stem Cells - cytology
Stromal Cells - cytology
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Summary:We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passage cultures from 2 of 7 individuals. The percent of aberrant cells in cultures from different individuals varied from 1.5 to 5.95 per 100 cells, which attested to karyotype instability. These data substantiate the need for cytogenetic control of cells before their transplantation into donor organism and further investigation of chromosome variability in stem cells.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-007-0031-0