Chromosome variability of human multipotent mesenchymal stromal cells

We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passag...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2007-01, Vol.143 (1), p.122-126
Main Authors: Bochkov, N P, Voronina, E S, Kosyakova, N V, Liehr, T, Rzhaninova, A A, Katosova, L D, Platonova, V I, Gol'dshtein, D V
Format: Article
Language:English
Subjects:
Adult
Cell Culture Techniques
Cells, Cultured
Chromosome Aberrations
Chromosomes, Human
Cytogenetic Analysis
Humans
Karyotyping
Mesenchymal Stem Cells - cytology
Middle Aged
Multipotent Stem Cells - cytology
Stromal Cells - cytology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c358t-ec747159f99f95999457b8c99442e7ceffb90289626a052cf26ae6aa55e52a553
cites cdi_FETCH-LOGICAL-c358t-ec747159f99f95999457b8c99442e7ceffb90289626a052cf26ae6aa55e52a553
container_end_page 126
container_issue 1
container_start_page 122
container_title Bulletin of experimental biology and medicine
container_volume 143
creator Bochkov, N P
Voronina, E S
Kosyakova, N V
Liehr, T
Rzhaninova, A A
Katosova, L D
Platonova, V I
Gol'dshtein, D V
description We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passage cultures from 2 of 7 individuals. The percent of aberrant cells in cultures from different individuals varied from 1.5 to 5.95 per 100 cells, which attested to karyotype instability. These data substantiate the need for cytogenetic control of cells before their transplantation into donor organism and further investigation of chromosome variability in stem cells.
doi_str_mv 10.1007/s10517-007-0031-0
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_745902791</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68511102</sourcerecordid><originalsourceid>FETCH-LOGICAL-c358t-ec747159f99f95999457b8c99442e7ceffb90289626a052cf26ae6aa55e52a553</originalsourceid><addsrcrecordid>eNp9UV1LBCEUlShq2_oBvcTQQz1NeZ1x1MdYtg9Y6KWexTGHnWUcN3WC_fcpuxD0EKjniuccrvcgdAX4HjBmDwEwBVamMu0KSnyEZkBZVXJC4BjNcH6pOedn6DyETb7iBk7RGXAMAhM-Q8vF2jvrgrOm-Fa-V20_9HFXuK5YT1aNhZ2G2G9dNGMsrAlm1OudVUMRYtIl1GYYwgU66dQQzOUB5-jjafm-eClXb8-vi8dVqSvKY2k0qxlQ0Ym0qBCipqzlOmFNDNOm69rclWhIozAluktoGqUoNZSks5qju73v1ruvyYQobR9yB2o0bgqS1TQZMAGJefsvs-EUADBJxJs_xI2b_Jh-IQlUdZovwYkEe5L2LgRvOrn1vVV-JwHLHIXcRyFzmaOQWXN9MJ5aaz5_FYfZVz_n7oMn</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213451720</pqid></control><display><type>article</type><title>Chromosome variability of human multipotent mesenchymal stromal cells</title><source>Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List</source><creator>Bochkov, N P ; Voronina, E S ; Kosyakova, N V ; Liehr, T ; Rzhaninova, A A ; Katosova, L D ; Platonova, V I ; Gol'dshtein, D V</creator><creatorcontrib>Bochkov, N P ; Voronina, E S ; Kosyakova, N V ; Liehr, T ; Rzhaninova, A A ; Katosova, L D ; Platonova, V I ; Gol'dshtein, D V</creatorcontrib><description>We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passage cultures from 2 of 7 individuals. The percent of aberrant cells in cultures from different individuals varied from 1.5 to 5.95 per 100 cells, which attested to karyotype instability. These data substantiate the need for cytogenetic control of cells before their transplantation into donor organism and further investigation of chromosome variability in stem cells.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-007-0031-0</identifier><identifier>PMID: 18019028</identifier><identifier>CODEN: BEXBAN</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Adult ; Cell Culture Techniques ; Cells, Cultured ; Chromosome Aberrations ; Chromosomes, Human ; Cytogenetic Analysis ; Humans ; Karyotyping ; Mesenchymal Stem Cells - cytology ; Middle Aged ; Multipotent Stem Cells - cytology ; Stromal Cells - cytology</subject><ispartof>Bulletin of experimental biology and medicine, 2007-01, Vol.143 (1), p.122-126</ispartof><rights>Springer Science+Business Media, Inc. 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-ec747159f99f95999457b8c99442e7ceffb90289626a052cf26ae6aa55e52a553</citedby><cites>FETCH-LOGICAL-c358t-ec747159f99f95999457b8c99442e7ceffb90289626a052cf26ae6aa55e52a553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18019028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bochkov, N P</creatorcontrib><creatorcontrib>Voronina, E S</creatorcontrib><creatorcontrib>Kosyakova, N V</creatorcontrib><creatorcontrib>Liehr, T</creatorcontrib><creatorcontrib>Rzhaninova, A A</creatorcontrib><creatorcontrib>Katosova, L D</creatorcontrib><creatorcontrib>Platonova, V I</creatorcontrib><creatorcontrib>Gol'dshtein, D V</creatorcontrib><title>Chromosome variability of human multipotent mesenchymal stromal cells</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><description>We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passage cultures from 2 of 7 individuals. The percent of aberrant cells in cultures from different individuals varied from 1.5 to 5.95 per 100 cells, which attested to karyotype instability. These data substantiate the need for cytogenetic control of cells before their transplantation into donor organism and further investigation of chromosome variability in stem cells.</description><subject>Adult</subject><subject>Cell Culture Techniques</subject><subject>Cells, Cultured</subject><subject>Chromosome Aberrations</subject><subject>Chromosomes, Human</subject><subject>Cytogenetic Analysis</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Middle Aged</subject><subject>Multipotent Stem Cells - cytology</subject><subject>Stromal Cells - cytology</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9UV1LBCEUlShq2_oBvcTQQz1NeZ1x1MdYtg9Y6KWexTGHnWUcN3WC_fcpuxD0EKjniuccrvcgdAX4HjBmDwEwBVamMu0KSnyEZkBZVXJC4BjNcH6pOedn6DyETb7iBk7RGXAMAhM-Q8vF2jvrgrOm-Fa-V20_9HFXuK5YT1aNhZ2G2G9dNGMsrAlm1OudVUMRYtIl1GYYwgU66dQQzOUB5-jjafm-eClXb8-vi8dVqSvKY2k0qxlQ0Ym0qBCipqzlOmFNDNOm69rclWhIozAluktoGqUoNZSks5qju73v1ruvyYQobR9yB2o0bgqS1TQZMAGJefsvs-EUADBJxJs_xI2b_Jh-IQlUdZovwYkEe5L2LgRvOrn1vVV-JwHLHIXcRyFzmaOQWXN9MJ5aaz5_FYfZVz_n7oMn</recordid><startdate>200701</startdate><enddate>200701</enddate><creator>Bochkov, N P</creator><creator>Voronina, E S</creator><creator>Kosyakova, N V</creator><creator>Liehr, T</creator><creator>Rzhaninova, A A</creator><creator>Katosova, L D</creator><creator>Platonova, V I</creator><creator>Gol'dshtein, D V</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200701</creationdate><title>Chromosome variability of human multipotent mesenchymal stromal cells</title><author>Bochkov, N P ; Voronina, E S ; Kosyakova, N V ; Liehr, T ; Rzhaninova, A A ; Katosova, L D ; Platonova, V I ; Gol'dshtein, D V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-ec747159f99f95999457b8c99442e7ceffb90289626a052cf26ae6aa55e52a553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Cell Culture Techniques</topic><topic>Cells, Cultured</topic><topic>Chromosome Aberrations</topic><topic>Chromosomes, Human</topic><topic>Cytogenetic Analysis</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Middle Aged</topic><topic>Multipotent Stem Cells - cytology</topic><topic>Stromal Cells - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bochkov, N P</creatorcontrib><creatorcontrib>Voronina, E S</creatorcontrib><creatorcontrib>Kosyakova, N V</creatorcontrib><creatorcontrib>Liehr, T</creatorcontrib><creatorcontrib>Rzhaninova, A A</creatorcontrib><creatorcontrib>Katosova, L D</creatorcontrib><creatorcontrib>Platonova, V I</creatorcontrib><creatorcontrib>Gol'dshtein, D V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Bulletin of experimental biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bochkov, N P</au><au>Voronina, E S</au><au>Kosyakova, N V</au><au>Liehr, T</au><au>Rzhaninova, A A</au><au>Katosova, L D</au><au>Platonova, V I</au><au>Gol'dshtein, D V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromosome variability of human multipotent mesenchymal stromal cells</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><addtitle>Bull Exp Biol Med</addtitle><date>2007-01</date><risdate>2007</risdate><volume>143</volume><issue>1</issue><spage>122</spage><epage>126</epage><pages>122-126</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><coden>BEXBAN</coden><abstract>We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passage cultures from 2 of 7 individuals. The percent of aberrant cells in cultures from different individuals varied from 1.5 to 5.95 per 100 cells, which attested to karyotype instability. These data substantiate the need for cytogenetic control of cells before their transplantation into donor organism and further investigation of chromosome variability in stem cells.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>18019028</pmid><doi>10.1007/s10517-007-0031-0</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0007-4888
ispartof Bulletin of experimental biology and medicine, 2007-01, Vol.143 (1), p.122-126
issn 0007-4888
1573-8221
language eng
recordid cdi_proquest_miscellaneous_745902791
source Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List
subjects Adult
Cell Culture Techniques
Cells, Cultured
Chromosome Aberrations
Chromosomes, Human
Cytogenetic Analysis
Humans
Karyotyping
Mesenchymal Stem Cells - cytology
Middle Aged
Multipotent Stem Cells - cytology
Stromal Cells - cytology
title Chromosome variability of human multipotent mesenchymal stromal cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T20%3A53%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chromosome%20variability%20of%20human%20multipotent%20mesenchymal%20stromal%20cells&rft.jtitle=Bulletin%20of%20experimental%20biology%20and%20medicine&rft.au=Bochkov,%20N%20P&rft.date=2007-01&rft.volume=143&rft.issue=1&rft.spage=122&rft.epage=126&rft.pages=122-126&rft.issn=0007-4888&rft.eissn=1573-8221&rft.coden=BEXBAN&rft_id=info:doi/10.1007/s10517-007-0031-0&rft_dat=%3Cproquest_cross%3E68511102%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c358t-ec747159f99f95999457b8c99442e7ceffb90289626a052cf26ae6aa55e52a553%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=213451720&rft_id=info:pmid/18019028&rfr_iscdi=true