Immunodiagnosis of Chagas disease: Synthesis of three latex–protein complexes containing different antigens of Trypanosoma cruzi

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2010-05, Vol.77 (1), p.12-17
• Main Authors: Gonzalez, Verónica D.G., Garcia, Valeria S., Vega, Jorge R., Marcipar, Ivan S., Meira, Gregorio R., Gugliotta, Luis M.
• Format: Article
• Language: English
• Subjects: Antigens; Antigens, Protozoan - analysis; Chagas disease; Chagas Disease - diagnosis; Humans; Hydrogen-Ion Concentration; Immunoassays; Isoelectric Point; Joining; Latex - chemistry; Latex–protein complex; Polystyrene resins; Proteins; Recombinant; Recombinant proteins; Recombinant Proteins - chemical synthesis; Surface chemistry; Synthesis; Trypanosoma cruzi; Trypanosoma cruzi - immunology
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2009.12.021

This article describes the physical adsorption and the chemical coupling of 3 antigenic proteins of Trypanosoma cruzi onto polystyrene (PS) based latexes to be used as novel immunodiagnosis reagents for detecting the Chagas disease. The coupled proteins were a homogenate of T. cruzi, or a recombinant protein (either Ag36 or CP1). With the homogenate, between 30 and 60% of the total-linked protein was chemically coupled, showing a small dependence with the pH. For Ag36 and CP1, around 90% of the total-linked protein was chemically coupled, with a maximum coupling at pH 5 ( i.e., close to the isoelectric point). The chemical coupling of CP1 was less affected by the pH than the coupling of Ag36.