Magnetically enhanced high-specificity virus detection using bio-activated magnetic nanoparticles with antibodies as labeling markers

• Published in: Journal of virological methods 2010-03, Vol.164 (1), p.14-18
• Main Authors: Yang, S.Y., Wang, W.C., Lan, C.B., Chen, C.H., Chieh, J.J., Horng, H.E., Hong, Chin-Yih, Yang, H.C., Tsai, C.P., Yang, C.Y., Cheng, I.C., Chung, W.C.
• Format: Article
• Language: English
• Subjects: Antibodies; Antigens, Viral - analysis; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; High specificity; Immunoassay - methods; Magnetic field; Magnetic nanoparticles; Magnetics; Microbiology; Nanoparticles; Sensitivity and Specificity; Techniques used in virology; Virology; Virology - methods; Virus; Viruses - isolation & purification
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2009.11.016

This study describes magnetically driven suppression of cross-reactions among molecules. First, the magnetic nanoparticles are coated with bio-probes and dispersed in liquid. The bio-probes can then bind with homologous or heterologous bio-targets. When alternating-current (ac) magnetic fields are applied, magnetic nanoparticles rotate driven by ac magnetic fields. Thus, the bio-targets bound on the surface of magnetic nanoparticles experience a centrifugal force. The centrifugal force can be manipulated by adjusting the angular frequency of the rotating magnetic nanoparticles. The angular frequency is determined by the applied ac magnetic field frequency. Since the binding force for good binding is much higher than that of poor binding, frequency manipulation is needed for the centrifugal force to be higher than the poor-binding force but lower than the good-binding force. Therefore, poor binding which contributes to cross reactions between molecules can be suppressed efficiently by control of the ac magnetic field frequency.