A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA

We developed an enzyme-based method to certify quadruplex formations in biologically important RNAs. Here, we developed a reverse transcriptase based method ( RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop a...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2010-04, Vol.20 (7), p.2350-2353
Main Authors: Hagihara, Masaki, Yoneda, Keisuke, Yabuuchi, Hiroaki, Okuno, Yasushi, Nakatani, Kazuhiko
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
G-Quadruplexes
Humans
mRNA
Nucleic acids
Reverse transcriptase
RNA quadruplex
RNA, Messenger - genetics
Rna, ribonucleoproteins
RNA-Directed DNA Polymerase - metabolism
Untranslated Regions
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Summary:We developed an enzyme-based method to certify quadruplex formations in biologically important RNAs. Here, we developed a reverse transcriptase based method ( RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop assay, we also revealed a plausible structural polymorphism in biologically important RNAs. The RTase stop assay would provide helpful insight into RNA quadruplex structures and functions, together with other analytical methods, including various footprinting techniques.
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2010.01.158