Saccharification of ionic liquid pretreated biomass with commercial enzyme mixtures

The performance of Spezyme CP, a commercial cellulase system, and Primafast Luna CL, a textile bio-finishing enzyme, on hydrolysis of substrates following ionic liquid pretreatment was evaluated. Ionic liquid pretreatment of lignocellulosic biomass produces amorphous cellulose with little residual c...

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Bibliographic Details
Published in:Bioresource technology 2010-05, Vol.101 (10), p.3561-3566
Main Authors: Samayam, Indira P., Schall, Constance A.
Format: Article
Language:English
Subjects:
1-n-ethyl-3-methyl imidazolium acetate
Biological and medical sciences
Biomass
Carbohydrate Metabolism
Cellulase
cellulases
cellulose
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
enzymatic hydrolysis
Enzyme saccharification
enzyme substrates
Enzymes - metabolism
Fundamental and applied biological sciences. Psychology
glucans
Hydrolysis
Ionic liquid pretreatment
lignocellulose
Panicum virgatum
Poplar
Populus
saccharification
Switchgrass
X-Ray Diffraction
xylanases
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Summary:The performance of Spezyme CP, a commercial cellulase system, and Primafast Luna CL, a textile bio-finishing enzyme, on hydrolysis of substrates following ionic liquid pretreatment was evaluated. Ionic liquid pretreatment of lignocellulosic biomass produces amorphous cellulose with little residual crystallinity and enhances its saccharification. The high crystallinity of native cellulose poses an impediment to enzyme hydrolysis of the cellulosic portion of biomass. Target substrates included poplar, switchgrass and Avicel, a highly crystalline cellulose substrate. Spezyme was found to hydrolyze glucan and xylan components completely in 24 h with modest enzyme loadings. Primafast was found to principally hydrolyze glucan components of biomass. Xylanases added to the Primafast enzyme mixture appear to act synergistically to improve glucan hydrolysis with the poplar substrate.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2009.12.066