Medium optimization for a novel 58 kDa dimeric keratinase from Bacillus licheniformis ER-15: Biochemical characterization and application in feather degradation and dehairing of hides

A novel dimeric 58 kDa keratinase is reported from Bacillus licheniformis ER-15. The bacterium produced 244 U/ml keratinase in 48 h which was increased by eight fold (1962 U/ml) after medium optimization by one-variable-at-a-time and response surface methodology. Enzyme was concentrated by ultrafilt...

Full description

Saved in:
Bibliographic Details
Published in:Bioresource technology 2010-08, Vol.101 (15), p.6103-6110
Main Authors: TIWARY, Ekta, GUPTA, Rani
Format: Article
Language:English
Subjects:
Animals
Bacillus - enzymology
Bacillus licheniformis
Biological and medical sciences
Biotechnology
Cell Culture Techniques - methods
Dimerization
Enzyme Activation
Enzyme Stability
Feathers - chemistry
Fundamental and applied biological sciences. Psychology
Hair - chemistry
Molecular Weight
Peptide Hydrolases - chemistry
Skin - chemistry
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A novel dimeric 58 kDa keratinase is reported from Bacillus licheniformis ER-15. The bacterium produced 244 U/ml keratinase in 48 h which was increased by eight fold (1962 U/ml) after medium optimization by one-variable-at-a-time and response surface methodology. Enzyme was concentrated by ultrafiltration followed by acetone precipitation and purified by gel filtration chromatography. It had subunit of 30 and 28 kDa and pI of 8.4. Enzyme was maximally active at pH 11 and 70 degrees C. It hydrolyzed various complex proteins viz. haemoglobin, feather, hooves, fibrin and meat protein. It was a thiol activated serine protease and 6.25-fold enhancement in activity was observed in presence of 5mM mercaptoethanol. Nearly 1200 U keratinase degraded 1.5 g feather in 12h at pH 8, 50 degrees C in redox free environment. This enzyme also dehaired buffalo hide within 16 h in presence of 3% Ca (OH)(2).
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2010.02.090