A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet

We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8–1.6 ng of φX174 D...

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Bibliographic Details
Published in:Analytical biochemistry 2010-07, Vol.402 (1), p.99-101
Main Authors: Cong, Wei-Tao, Zhu, Zhong-Xin, He, Hong-Zhang, Jin, Yan, Jiang, Cheng-Xi, Choi, Jung-Kap, Jin, Li-Tai, Li, Xiao-Kun
Format: Article
Language:English
Subjects:
DNA - analysis
Electrophoresis, Polyacrylamide Gel - methods
Rosaniline Dyes
Sensitivity and Specificity
Staining and Labeling - economics
Staining and Labeling - methods
Viral Proteins - genetics
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Summary:We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8–1.6 ng of φX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2010.03.017