Multi-transcript expression patterns in the gastrolith disk and the hypodermis of the crayfish Cherax quadricarinatus at premolt

In the crustacean Cherax quadricarinatus, alterations of multi-transcript expression patterns between intermolt and late premolt stages were identified in the hypodermis and in the gastrolith disk via a cDNA microarray. The gastrolith disk is a specialized epithelium forming the gastroliths at premo...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. Part D, Genomics & proteomics Genomics & proteomics, 2010-06, Vol.5 (2), p.171-177
Main Authors: Yudkovski, Yana, Glazer, Lilah, Shechter, Assaf, Reinhardt, Richard, Chalifa-Caspi, Vered, Sagi, Amir, Tom, Moshe
Format: Article
Language:English
Subjects:
Animals
Arthropods
Astacoidea - genetics
Astacoidea - growth & development
Astacoidea - metabolism
Cambaridae
Cherax quadricarinatus
Chitin deacetylase
Crustaceans
Gastrolith disk
Gene Expression Profiling
Gene Expression Regulation, Developmental
Hypodermis
Molt cycle
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
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Summary:In the crustacean Cherax quadricarinatus, alterations of multi-transcript expression patterns between intermolt and late premolt stages were identified in the hypodermis and in the gastrolith disk via a cDNA microarray. The gastrolith disk is a specialized epithelium forming the gastroliths at premolt. The gastroliths are deposits of calcium carbonate derived from the digested cuticle contributing the mineral to the newly formed exoskeleton at postmolt. The late premolt stage was characterized by a dramatic general up-regulation of genes in the gastrolith disk. This phenomenon is explained by the gastrolith disk function rapid formation of the relatively large gastrolith during a short period of time. Besides genes of general importance for this dramatic change, three genes related to the chitin–protein–mineral structure were identified. The cDNA and the deduced protein of the novel one of them, the chitin deacetylase 1 (Cq-CDA1) was fully characterized and its resemblance to already characterized structural proteins of the gastrolith matrix was described. Cq-CDA1 characteristics strongly indicate its participation in the gastrolith construction, although its protein product was not identified yet in the gastrolith. In addition, many differentially expressed genes with unknown function were elucidated. An unexpected milder down-regulation was observed in the hypodermis.
ISSN:1744-117X
1878-0407
DOI:10.1016/j.cbd.2010.03.010