CHARACTERIZATION OF A VIRUS ISOLATED FROM A CASE OF HUMAN INFECTIOUS HEPATITIS

A new viral agent, isolated from the serum of an infectious hepatitis patient and designated as Agent II-B, was extensively studied in In vitro and in vivo systems. Agent Il-B multiplied well in primary and serial animal cell cultures and in embryonated hen's eggs. Quantal and quantitative Infe...

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Bibliographic Details
Published in:American journal of epidemiology 1979-05, Vol.109 (5), p.572-581
Main Authors: WOODFORD, PAMELA C., HANSEN, P. ARNE, ROANE, PHILIP R., WARD, THOMAS G.
Format: Article
Language:English
Subjects:
Benzimidazoles - pharmacology
Benzyl Alcohols - pharmacology
Fluorescent Antibody Technique
Guanidines - pharmacology
Hemagglutination, Viral
hepatitis
Hepatitis A - microbiology
Humans
Hydrogen-Ion Concentration
Idoxuridine - pharmacology
infectious
Neutralization Tests
Picornaviridae - drug effects
Picornaviridae - isolation & purification
picornaviruses
Virus Cultivation
Virus Replication
viruses
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Summary:A new viral agent, isolated from the serum of an infectious hepatitis patient and designated as Agent II-B, was extensively studied in In vitro and in vivo systems. Agent Il-B multiplied well in primary and serial animal cell cultures and in embryonated hen's eggs. Quantal and quantitative Infectivity assays were performed In monolayers of African green monkey kidney cells. Effective concentrations of 5-iodo-2-deoxyuridine and guanidine hydrochloride did not inhibit the multiplication of Agent II-B, although 2-hydroxybenzylbenzimidazole was an effective inhibitor. Essential lipids were not detected. The diameter of the agent is 16–25 nm and its buoyant density in CsCI equilibrium density gradients was 1.35 gm/ml. Neutralization test results did not reveal antlgenlc relatedness between Agent II-B and known human picornaviruses. Apparently, this new viral agent is a picornavirus which possesses the capacity to multiply in unexpectedly diverse cell types.
ISSN:0002-9262
1476-6256
DOI:10.1093/oxfordjournals.aje.a112715