A stable chemiluminescent-labelled antibody for immunological assays

RADIOISOTOPES, in particular 125 I, have been used for many years as labels for monitoring the distribution of reagents in immunological assay systems. Satisfactory labelled derivatives are not always easily produced as iodination frequently produces severe molecular disruption. Moreover, iodinated...

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Bibliographic Details
Published in:Nature (London) 1979-06, Vol.279 (5714), p.646-647
Main Authors: SIMPSON, J. S. A, CAMPBELL, A. K, RYALL, M. E. T, WOODHEAD, J. S
Format: Article
Language:English
Subjects:
Animals
Antibodies, Anti-Idiotypic
Antibody Specificity
Chemical Phenomena
Chemistry
Humanities and Social Sciences
Immunoassay - methods
Immunoglobulin G - analysis
letter
Luminescent Measurements
Luminol
multidisciplinary
Pyridazines
Science
Science (multidisciplinary)
Sheep
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Summary:RADIOISOTOPES, in particular 125 I, have been used for many years as labels for monitoring the distribution of reagents in immunological assay systems. Satisfactory labelled derivatives are not always easily produced as iodination frequently produces severe molecular disruption. Moreover, iodinated proteins have a limited shelf life and sometimes require extended counting times for accurate quantitation. Recently, non-radioactive labels such as bacteriophages 1,2 , enzymes 3,4 , stable free radicals 5 and fluorescent groups 6 have been used in attempts to overcome some of these problems. We describe here the successful labelling of antibodies to rabbit IgG with a chemiluminescent molecule and the development of an immunological assay using chemiluminescence as a means of monitoring reagent distribution.
ISSN:0028-0836
1476-4687
DOI:10.1038/279646a0