Neonatal cardiac mitochondria and ischemia/reperfusion injury

Postnatal maturation of the heart is characterized by decreasing tolerance to ischemia/reperfusion (I/R) injury associated with significant changes in mitochondrial function. The aim of this study is to test the hypothesis that the role of the mitochondrial membrane permeability transition pore (MPT...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular biochemistry 2010-02, Vol.335 (1-2), p.147-153
Main Authors: Milerova, Marie, Charvatova, Zuzana, Skarka, Libor, Ostadalova, Ivana, Drahota, Zdenek, Fialova, Martina, Ostadal, Bohuslav
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Biochemistry
Biomedical and Life Sciences
Calcium
Cardiology
Comparative studies
Infants (Newborn)
Lactones - pharmacology
Life Sciences
Medical Biochemistry
Mitochondria, Heart - metabolism
Mitochondrial DNA
Mitochondrial Membrane Transport Proteins - metabolism
Neonatal care
Oncology
Permeability
Rats
Rats, Wistar
Reperfusion Injury - metabolism
Rodents
Spiro Compounds - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Postnatal maturation of the heart is characterized by decreasing tolerance to ischemia/reperfusion (I/R) injury associated with significant changes in mitochondrial function. The aim of this study is to test the hypothesis that the role of the mitochondrial membrane permeability transition pore (MPTP) in the I/R injury differs in the neonatal and in the adult heart. For this purpose, the effect of blockade of MPTP on the degree of I/R injury and the sensitivity of MPTP to swelling-inducing agents was compared in hearts from neonatal (7 days old) and adult (90 days old) Wistar rats. It was found that the release of NAD⁺ from the perfused heart induced by I/R can be prevented by sanglifehrin A (SfA) only in the adult myocardium; SfA had no protective effect in the neonatal heart. Furthermore, the extent of Ca-induced swelling of mitochondria from neonatal rats was significantly lower than that from the adult animals; mitochondria from neonatal rats were more resistant at higher concentrations of calcium. In addition, not only the extent but also the rate of calcium-induced swelling was about twice higher in adult than in neonatal mitochondria. The results support the idea that lower sensitivity of the neonatal MPTP to opening may be involved in the mechanism of the higher tolerance of the neonatal heart to I/R injury.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-009-0251-x