Complete genome sequence of a tobacco isolate of the tobacco vein banding mosaic virus strain prevailing in China

• Published in: Archives of virology 2010-02, Vol.155 (2), p.293-295
• Main Authors: Wang, H.-Y, Zhu, T.-S, Cui, T.-T, Hou, S.-S, Yin, X, Li, Xiang-Dong, Lei, L.-P, Zhu, X.-P
• Format: Article
• Language: English
• Subjects: Amino acids; Annotated Sequence Record; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; China; Cluster Analysis; Fundamental and applied biological sciences. Psychology; Genome, Viral; Genomes; Genomics; Host range; Infectious Diseases; Medical Microbiology; Microbiology; Miscellaneous; Molecular Sequence Data; Nicotiana - virology; Nicotiana tabacum; Nucleotide sequence; Nucleotides; Open Reading Frames; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - virology; Plant viruses; Plant viruses and viroids; Polyadenylation; Polyproteins - genetics; Potyvirus; Potyvirus - genetics; Potyvirus - isolation & purification; RNA, Messenger; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Tobacco; Tobacco vein banding mosaic virus; Tomato mosaic virus; Viral Proteins - genetics; Virology
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-009-0570-y

Tobacco vein banding mosaic virus (TVBMV) is a species of the largest plant virus genus Potyvirus. Its incidence has been increasing in Chinese tobacco-growing area. TVBMV isolates can be clustered into three genetic groups that are corresponding with their geographical origin. We have reported the complete genomic sequence of TVBMV isolate YND with unique NIb/CP cleavage site. Here, we determined and analyzed the complete genomic sequence of isolate HN39, which was collected from tobacco in Henan Province and represented Chinese prevalent strain of TVBMV. HN39 has similar host range with YND, but induce mild vein banding symptom in Nicotiana tabacum cv. Samsun. The genome of TVBMV-HN39 is composed of 9,570 nucleotides, excluding the poly(A) tail. It contains a large ORF of 9,240 nucleotides and encode a polyprotein of 3,079 amino acids. The putative NIa-Pro cleavage site for NIb/CP is Q/G. The identities between the complete genomes of isolates HN39 and YND were 90.0% at nucleotide level and 95.4% at amino acid level. As for other potyviruses, HN39 shared the highest identity with wild tomato mosaic virus (WTMV) at complete genomic level, while different genes shared the highest identities with different potyviruses. This is the second complete genomic sequence of TVBMV reported.